PMID:8159699

Citation	Martasek, P, Camadro, JM, Delfau-Larue, MH, Dumas, JB, Montagne, JJ, de Verneuil, H, Labbe, P and Grandchamp, B (1994) Molecular cloning, sequencing, and functional expression of a cDNA encoding human coproporphyrinogen oxidase. Proc. Natl. Acad. Sci. U.S.A. 91:3024-8
Abstract	Coproporphyrinogen oxidase (EC 1.3.3.3) catalyzes the sixth step in the heme biosynthetic pathway, the oxidation of coproporphyrinogen III to protoporphyrinogen IX. The activity of this enzyme is deficient in the disease hereditary coproporphyria. The sequence of the cDNA and predicted amino acid sequence of the human coproporphyrinogen oxidase are presented. The human protein sequence contains a region completely homologous to that we obtained by sequencing an 11-amino acid peptide fragment from purified murine liver coproporphyrinogen oxidase. Results of Southern blotting were consistent with the presence of a single human coproporphyrinogen oxidase gene, and Northern blotting demonstrated one transcript of similar size in erythroid and nonerythroid cell lines. Expression of the cDNA coding for the putative mature human coproporphyrinogen oxidase in Escherichia coli resulted in a 17-fold increase in coproporphyrinogen activity over endogenous activity.
Links	PubMed PMC43507
Keywords	Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; Coproporphyrinogen Oxidase/genetics; DNA Primers/chemistry; DNA, Complementary/genetics; Gene Expression; Genes; Humans; Mice; Molecular Sequence Data; RNA, Messenger/genetics; Restriction Mapping
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:HEM6	involved_in	GO:0006783: heme biosynthetic process	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete

References

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