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PMID:8104433

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Citation

Baude, A, Nusser, Z, Roberts, JD, Mulvihill, E, McIlhinney, RA and Somogyi, P (1993) The metabotropic glutamate receptor (mGluR1 alpha) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction. Neuron 11:771-87

Abstract

An antiserum to mGluR1 alpha labeled a 160 kd protein in immunoblots of membranes derived from rat brain or cells transfected with mGluR1 alpha. Immunoreactivity for mGluR1 alpha was present in discrete subpopulations of neurons. The GABAergic neurons of the cerebellar cortex were strongly immunoreactive; only some Golgi cells were immunonegative. Somatostatin/GABA-immunopositive cells in the neocortex and hippocampus were enriched in mGluR1 alpha. The hippocampal cells had spiny dendrites that were precisely codistributed with the local axon collaterals of pyramidal and granule cells. Electron microscopic immunometal detection of mGluR1 alpha showed a preferential localization at the periphery of the extensive postsynaptic densities of type 1 synapses in both the cerebellum and the hippocampus. The receptor was also present at sites in the dendritic and somatic membrane where synapses were not located.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Brain/cytology; Cell Line; Cerebellum/cytology; Cerebellum/ultrastructure; Electrophoresis, Polyacrylamide Gel; Hippocampus/cytology; Hippocampus/ultrastructure; Immune Sera; Immunohistochemistry/methods; Membrane Proteins/analysis; Membrane Proteins/isolation & purification; Microscopy, Immunoelectron/methods; Molecular Sequence Data; Molecular Weight; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/isolation & purification; Neurons/cytology; Neurons/ultrastructure; Oligopeptides/chemical synthesis; Oligopeptides/immunology; Organ Specificity; Rats; Receptors, Glutamate/analysis; Recombinant Proteins/analysis; Somatostatin/analysis; Synaptic Membranes/ultrastructure; Transfection; gamma-Aminobutyric Acid/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:GRM1

located_in

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRM1

located_in

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRM1

located_in

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:GRM1

located_in

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRM1

located_in

GO:0099055: integral component of postsynaptic membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:GRM1

located_in

GO:0099055: integral component of postsynaptic membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0099055: integral component of postsynaptic membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0099055: integral component of postsynaptic membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRM1

part_of

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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