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PMID:8100228
| Citation |
Caprioglio, DR, Padilla, C and Werner-Washburne, M (1993) Isolation and characterization of AAP1. A gene encoding an alanine/arginine aminopeptidase in yeast. J. Biol. Chem. 268:14310-5 |
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| Abstract |
The yeast AAP1 gene, encoding a putative amino-peptidase, was isolated based on its ability to suppress the temperature-sensitive growth on nonfermentable carbon sources of spr5, a stationary phase regulatory mutant. AAP1 was physically mapped to chromosome VIII between PUT2 and CUP1. Sequence analysis of the AAP1 gene showed a 1581-nucleotide open reading frame capable of encoding a 59-kilodalton protein. The protein encoded by this open reading frame exhibits approximately 40% sequence identity to human, rat, and mouse aminopeptidases. In limited regions, sequence identity between Aap1 and the mammalian aminopeptidases ranges from 53% to 93%. Insertional inactivation of the AAP1 gene resulted in a decrease in glycogen accumulation and the loss of the major band of arginine/alanine aminopeptidase activity. Strains carrying the AAP1 gene on a high copy plasmid show an increase in the major arginine/alanine aminopeptidase activity, a dramatic increase in glycogen accumulation, and an increase in transcription from a vector carrying lacZ fused to the promoter of a gene (SSA3) expressed during post-diauxic and stationary phases of the culture cycle. We conclude that although the AAP1 gene is not essential for viability, the Aap1 protein positively affects glycogen accumulation in yeast. |
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| Keywords |
Amino Acid Sequence; Aminopeptidases/biosynthesis; Aminopeptidases/genetics; Animals; Antigens, CD13; Base Sequence; Chromosome Mapping; Chromosomes, Fungal; DNA, Fungal/genetics; Genes, Fungal; Genetic Complementation Test; Humans; Mice; Molecular Sequence Data; Open Reading Frames; Phenotype; RNA, Messenger/metabolism; Rats; Recombinant Fusion Proteins/biosynthesis; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Sequence Homology, Amino Acid; beta-Galactosidase/biosynthesis; beta-Galactosidase/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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