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PMID:8083202

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Citation

Chen, J, Engle, SJ, Seilhamer, JJ and Tischfield, JA (1994) Cloning and characterization of novel rat and mouse low molecular weight Ca(2+)-dependent phospholipase A2s containing 16 cysteines. J. Biol. Chem. 269:23018-24

Abstract

A novel rat 4.4-kilobase (kb) cDNA encoding a low molecular weight Ca(2+)-dependent phospholipase A2 (PLA2) and its murine homologue were cloned. The rat and mouse cDNA predict a mature protein of 130 amino acids (M(r) = 14, 763) preceded by a 28-amino acid prepro-peptide. The deduced amino acid sequences encode a protein containing 16 cysteines which distinguishes them from both mammalian group I and II PLA2s and the recently described group of mammalian PLA2s containing 12 cysteines. A rat RNA blot hybridized with the rat cDNA exhibited an abundant 2.3-kb and a less abundant 5-kb transcript in testis. When the rat cDNA was expressed using an Epstein-Barr virus-based vector in human 293s cells, PLA2 activity accumulated in the culture medium. Conditioned medium optimally hydrolyzed the phospholipids of [1-14C]oleate-labeled Escherichia coli at neutral to alkaline pH with 1-7 mM Ca2+. In assays with individual substrates, L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol was hydrolyzed more efficiently than L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, or L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Calcium/metabolism; Cloning, Molecular; Cysteine/analysis; DNA, Complementary; Exons; Gene Expression Regulation, Enzymologic; Humans; Mice; Molecular Sequence Data; Molecular Weight; Phospholipases A/chemistry; Phospholipases A/genetics; Phospholipases A/metabolism; Phospholipases A2; Rats; Sequence Homology, Amino Acid; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:PA2GC

enables

GO:0047498: calcium-dependent phospholipase A2 activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:PA2GC

acts_upstream_of_or_within

GO:0009395: phospholipid catabolic process

ECO:0000266: sequence orthology evidence used in manual assertion

EMBL:U07798

P

Seeded From UniProt

complete

MOUSE:PA2GC

enables

GO:0004623: phospholipase A2 activity

ECO:0000266: sequence orthology evidence used in manual assertion

EMBL:U07798

F

Seeded From UniProt

complete


See also

References

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