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PMID:8035824

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Citation

Dechesne, CA, Wei, Q, Eldridge, J, Gannoun-Zaki, L, Millasseau, P, Bougueleret, L, Caterina, D and Paterson, BM (1994) E-box- and MEF-2-independent muscle-specific expression, positive autoregulation, and cross-activation of the chicken MyoD (CMD1) promoter reveal an indirect regulatory pathway. Mol. Cell. Biol. 14:5474-86

Abstract

Members of the MyoD family of gene-regulatory proteins (MyoD, myogenin, myf5, and MRF4) have all been shown not only to regulate the transcription of numerous muscle-specific genes but also to positively autoregulate and cross activate each other's transcription. In the case of muscle-specific genes, this transcriptional regulation can often be correlated with the presence of a DNA consensus in the regulatory region CANNTG, known as an E box. Little is known about the regulatory interactions of the myogenic factors themselves; however, these interactions are thought to be important for the activation and maintenance of the muscle phenotype. We have identified the minimal region in the chicken MyoD (CMD1) promoter necessary for muscle-specific transcription in primary cultures of embryonic chicken skeletal muscle. The CMD1 promoter is silent in primary chick fibroblast cultures and in muscle cell cultures treated with the thymidine analog bromodeoxyuridine. However, CMD1 and chicken myogenin, as well as, to a lesser degree, chicken Myf5 and MRF4, expressed in trans can activate transcription from the minimal CMD1 promoter in these primary fibroblast cultures. Here we show that the CMD1 promoter contains numerous E-box binding sites for CMD1 and the other myogenic factors, as well as a MEF-2 binding site. Surprisingly, neither muscle-specific and the other myogenic factors, as well as a MEF-2 binding site. Surprisingly, neither muscle-specific expression, autoregulation, or cross activation depends upon the presence of of these E-box or MEF-2 binding sites in the CMD1 promoter. These results demonstrate that the autoregulation and cross activation of the chicken MyoD promoter through the putative direct binding of the myogenic basic helix-loop-helix regulatory factors is mediated through an indirect pathway that involves unidentified regulatory elements and/or ancillary factors.

Links

PubMed PMC359067

Keywords

Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Chickens; Cloning, Molecular; DNA-Binding Proteins/metabolism; Gene Expression Regulation; Genes; Helix-Loop-Helix Motifs; Molecular Sequence Data; Muscle Proteins/physiology; Muscles/physiology; MyoD Protein/genetics; Myogenic Regulatory Factor 5; Myogenic Regulatory Factors/physiology; Myogenin/physiology; Promoter Regions, Genetic; Trans-Activators; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MYF5

part_of

GO:0090575: RNA polymerase II transcription regulator complex

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q08856

C

Seeded From UniProt

complete

CHICK:MYOD1

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P16075

F

Seeded From UniProt

complete

CHICK:MYOD1

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYOD1

enables

GO:0043425: bHLH transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q70IK3

F

Seeded From UniProt

complete

CHICK:MYOD1

enables

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001158)

Seeded From UniProt

complete

CHICK:MYOG

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17920

F

Seeded From UniProt

complete

CHICK:MYOG

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYOG

enables

GO:0043425: bHLH transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q70IK3

F

Seeded From UniProt

complete

CHICK:MYOG

enables

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001158)

Seeded From UniProt

complete

CHICK:MYF6

part_of

GO:0090575: RNA polymerase II transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYF6

enables

GO:0043425: bHLH transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q70IK3

F

Seeded From UniProt

complete

CHICK:MYF6

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001158)

Seeded From UniProt

complete

CHICK:MYF5

part_of

GO:0090575: RNA polymerase II transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYF5

enables

GO:0043425: bHLH transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q70IK3

F

Seeded From UniProt

complete

CHICK:MYF5

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001158)

Seeded From UniProt

complete

CHICK:Q70IK3

part_of

GO:0090575: RNA polymerase II transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q70IK3

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P16075

F

Seeded From UniProt

complete

CHICK:Q70IK3

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17920

F

Seeded From UniProt

complete

CHICK:Q70IK3

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q08856

F

Seeded From UniProt

complete

CHICK:Q70IK3

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q01795

F

Seeded From UniProt

complete

CHICK:Q70IK3

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q70IK3

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_at:(SO:0001158)

Seeded From UniProt

complete

MOUSE:MEF2C

enables

GO:0000976: transcription cis-regulatory region binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYF5

involved_in

GO:0042693: muscle cell fate commitment

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYF5

enables

GO:0046982: protein heterodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYF5

part_of

GO:0090575: RNA polymerase II transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYF5

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYF6

part_of

GO:0090575: RNA polymerase II transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYF6

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYF6

enables

GO:0046982: protein heterodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYF6

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYF6

involved_in

GO:0042693: muscle cell fate commitment

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYOD1

enables

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYOD1

enables

GO:0046982: protein heterodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYOD1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYOD1

involved_in

GO:0042693: muscle cell fate commitment

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYOD1

part_of

GO:0005667: transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYOG

part_of

GO:0005667: transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYOG

involved_in

GO:0042693: muscle cell fate commitment

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYOG

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYOG

enables

GO:0046982: protein heterodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:MYOG

enables

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q70IK3

enables

GO:0046982: protein heterodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q70IK3

part_of

GO:0005667: transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q70IK3

contributes_to

GO:0070888: E-box binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q70IK3

part_of

GO:0090575: RNA polymerase II transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MEF2C

enables

GO:0044212: transcription regulatory region DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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