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PMID:8032679

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Citation

Furukawa, K, Inagaki, H, Naruge, T, Tabata, S, Tomida, T, Yamaguchi, A, Yoshikuni, M, Nagahama, Y and Hotta, Y (1994) cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association. Chromosome Res. 2:99-113

Abstract

It is well known that cytoskeleton and karyoskeleton proteins are associated with changes in cell shape and with the rearrangement of the dynamic structures involved in cell division and motility. In higher vertebrates, there are three major skeletal protein groups: microfilaments, microtubules and intermediate filaments, each representing a multigene family. Some of these skeletal proteins are expressed in a temporally- and spatially-specific fashion, and they establish cell-specific cytoplasmic and nucleoplasmic organization during development. Here we report the cDNA cloning of a novel 60 kDa skeletal protein from mouse spermatocytes, termed MNS 1 (meiosis-specific nuclear structural protein), whose computer-predicted protein configuration indicates long alpha-helical coiled-coil domains flanked by non-helical terminal domains. Functional characterization of MNS1 by ectopic expression in culture cells indicated that it is a detergent- and high salt-resistant skeletal protein which is involved in organization of the nuclear or perinuclear architecture. The MNS1 protein is specifically expressed at the pachytene stage during spermatogenesis, so that its function may involve the determination and maintenance of the appropriate nuclear morphology during meiotic prophase.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cells, Cultured; Cloning, Molecular; DNA, Complementary/genetics; Gene Expression Regulation; Intermediate Filaments/chemistry; Lamins; Male; Meiosis; Mice; Molecular Sequence Data; Nuclear Matrix/chemistry; Nuclear Proteins/biosynthesis; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Prophase; Protein Structure, Secondary; Recombinant Fusion Proteins/biosynthesis; Spermatocytes/chemistry; Testis/cytology; Testis/growth & development

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:LMNB1

located_in

GO:0005638: lamin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LMNB2

located_in

GO:0005638: lamin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LMNA

located_in

GO:0005638: lamin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MNS1

located_in

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MNS1

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LMNA

part_of

GO:0005638: lamin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MNS1

part_of

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MNS1

part_of

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LMNB1

part_of

GO:0005638: lamin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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