PMID:8012595

Citation	Völker, U, Engelmann, S, Maul, B, Riethdorf, S, Völker, A, Schmid, R, Mach, H and Hecker, M (1994) Analysis of the induction of general stress proteins of Bacillus subtilis. Microbiology (Reading, Engl.) 140 ( Pt 4):741-52
Abstract	In Bacillus subtilis stress proteins are induced in response to different environmental conditions such as heat shock, salt stress, glucose and oxygen limitation or oxidative stress. These stress proteins have been previously grouped into general stress proteins (Gsps) and heat-specific stress proteins (Hsps). In this investigation the N-terminal sequences of 13 stress proteins of B. subtilis were determined. The quantification of the mRNA and the analysis of the protein synthesis pattern support the initial hypothesis that the chaperones DnaK and GroEL are Hsps in B. subtilis. In contrast, the recently described proteins GsiB, Ctc and RsbW belong to a class of Gsps that are induced by various stresses including heat shock. The main part of the Gsps described in this study failed to be induced in the sigB deletion mutant ML6 in response to heat shock. However, all the five Hsps were induced in this mutant in response to heat shock. These data indicate that SigB plays a crucial role in the induction of general stress genes, but is dispensable for the induction of Hsps.
Links	PubMed
Keywords	Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Bacterial; Genes, Bacterial; Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/genetics; Molecular Sequence Data; Oxygen/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:RPSB		GO:0043620: regulation of DNA-dependent transcription in response to stress	ECO:0000315:		P		The synthesis of stress proteins in the sigB mutant ML6 during heat shock was analysed by two-dimensional protein electrophoresis(Fig. 5).	complete CACAO 2655
BACSU:RPSB	involved_in	GO:0043620: regulation of DNA-templated transcription in response to stress	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BACSU:RSBX	involved_in	GO:0009408: response to heat	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete
BACSU:RSBX		GO:0009408: response to heat	ECO:0000270:		P		Figure 4b.	complete CACAO 2656
BACSU:CH60		GO:0006950: response to stress	ECO:0000270:		P		Figure 4c.	complete
BACSU:CTC		GO:0006950: response to stress	ECO:0000270:		P		Figure 4b.	complete CACAO 2669

References

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