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PMID:8006065

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Citation

North, AJ, Gimona, M, Lando, Z and Small, JV (1994) Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell. Sci. 107 ( Pt 3):445-55

Abstract

Differentiated smooth muscle cells typically contain a mixture of muscle (alpha and gamma) and cytoplasmic (beta and gamma) actin isoforms. Of the cytoplasmic actins the beta-isoform is the more dominant, making up from 10% to 30% of the total actin complement. Employing an antibody raised against the N-terminal peptide specific to beta-actin, which labels only the beta-isoform on two-dimensional gel immunoblots, we have shown that this isoform has a restricted localisation in smooth muscle. Using double-label immunofluorescence and immunoelectron microscopy of ultrathin sections of chicken gizzard, beta-actin was localised in the dense bodies and in longitudinal channels linking consecutive dense bodies that were also occupied by desmin. It was additionally found in the membrane-associated dense plaques, but was excluded from the actomyosin-containing regions of the contractile apparatus. Taken together with earlier results these findings identify a cytoskeletal compartment containing intermediate filaments, cytoplasmic actin and the actin cross-linking protein filamin. Using an antibody specific only for muscle actin, labelling was found generally around the myosin filaments of the contractile apparatus, but was absent from the core of the dense bodies that contained beta-actin. Thus, if dense bodies act as dual-purpose anchorage sites, for the cytoskeletal actin and the contractile actin, the thin filaments of the contractile apparatus must be anchored at the periphery of the dense bodies. A model of the structural organisation of the cell is presented and the possible roles of the cytoskeleton are discussed.

Links

PubMed

Keywords

Actins/chemistry; Amino Acid Sequence; Animals; Antibody Specificity; Cells, Cultured; Chickens; Cytoplasm/chemistry; Gizzard; Microscopy, Immunoelectron; Molecular Conformation; Molecular Sequence Data; Muscle, Smooth/chemistry; Muscle, Smooth/cytology; Muscle, Smooth/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:F1P476

located_in

GO:0032982: myosin filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:O73665

located_in

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:O73665

located_in

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:DESM

located_in

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:DESM

located_in

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTN1

located_in

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

located_in

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

located_in

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

located_in

GO:0005856: cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

part_of

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

part_of

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTB

part_of

GO:0005856: cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ACTN1

part_of

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:DESM

part_of

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:DESM

part_of

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:O73665

part_of

GO:0005882: intermediate filament

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:O73665

part_of

GO:0097433: dense body

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

Notes

See also

References

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Internal error - GONUTS

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Internal error

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[37d0adeed114bbc5422e95d3] /wiki/index.php/PMID:8006065 Error from line 252 of /usr/local/github/wiki-extensions/wiki-extensions-hulab/AnnotationLinks/AnnotationLinks.php: Call to a member function getIdentifier() on null

Backtrace:

#0 /usr/local/github/wiki-extensions/wiki-extensions-hulab/AnnotationLinks/AnnotationLinks.php(70): AnnotationLinks::getQuickGOannotations(string, array)
#1 /var/www/html/go/wiki/includes/parser/Parser.php(3888): AnnotationLinks::renderAnnotations(NULL, array, Parser, PPFrame_DOM)
#2 /var/www/html/go/wiki/includes/parser/Preprocessor_DOM.php(1362): Parser->extensionSubstitution(array, PPFrame_DOM)
#3 /var/www/html/go/wiki/includes/parser/Parser.php(2942): PPFrame_DOM->expand(DOMElement, integer)
#4 /var/www/html/go/wiki/includes/parser/Parser.php(1293): Parser->replaceVariables(string)
#5 /var/www/html/go/wiki/includes/parser/Parser.php(443): Parser->internalParse(string)
#6 /var/www/html/go/wiki/includes/content/WikitextContent.php(323): Parser->parse(string, Title, ParserOptions, boolean, boolean, integer)
#7 /var/www/html/go/wiki/includes/content/AbstractContent.php(516): WikitextContent->fillParserOutput(Title, integer, ParserOptions, boolean, ParserOutput)
#8 /var/www/html/go/wiki/includes/jobqueue/jobs/RefreshLinksJob.php(231): AbstractContent->getParserOutput(Title, integer, ParserOptions, boolean)
#9 /var/www/html/go/wiki/includes/jobqueue/jobs/RefreshLinksJob.php(126): RefreshLinksJob->runForTitle(Title)
#10 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(296): RefreshLinksJob->run()
#11 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(193): JobRunner->executeJob(RefreshLinksJob, Wikimedia\Rdbms\LBFactorySimple, BufferingStatsdDataFactory, integer)
#12 /var/www/html/go/wiki/includes/MediaWiki.php(1002): JobRunner->run(array)
#13 /var/www/html/go/wiki/includes/MediaWiki.php(988): MediaWiki->triggerSyncJobs(integer, MediaWiki\Logger\LegacyLogger)
#14 /var/www/html/go/wiki/includes/MediaWiki.php(912): MediaWiki->triggerJobs()
#15 /var/www/html/go/wiki/includes/MediaWiki.php(727): MediaWiki->restInPeace(string, boolean)
#16 /var/www/html/go/wiki/includes/MediaWiki.php(750): MediaWiki->{closure}()
#17 /var/www/html/go/wiki/includes/MediaWiki.php(557): MediaWiki->doPostOutputShutdown(string)
#18 /var/www/html/go/wiki/index.php(42): MediaWiki->run()
#19 {main}

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