GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:7927664

From GONUTS
Jump to: navigation, search
Citation

Gessner, A, Schröppel, K, Will, A, Enssle, KH, Lauffer, L and Röllinghoff, M (1994) Recombinant soluble interleukin-4 (IL-4) receptor acts as an antagonist of IL-4 in murine cutaneous Leishmaniasis. Infect. Immun. 62:4112-7

Abstract

This study was performed to evaluate the soluble interleukin-4 receptor (sIL-4R) as a potential antagonist of interleukin-4 (IL-4) in an infectious disease. It is shown that antigen-triggered proliferation and cytokine secretion of Leishmania major-specific, cloned Th2 cells in vitro can be inhibited dose dependently by recombinant murine, but not control human, sIL-4R. In vivo, we found that endogenous synthesis of IL-4 mRNA is upregulated during the first week of infection, while an increase of IL-4R mRNA occurred later after infection of BALB/c mice with L. major. To interfere successfully with the IL-4 ligand-receptor interaction, we therefore chose to treat infected BALB/c mice with recombinant sIL-4R during the onset (e.g., days 0 to 7) of the immune response. Treatment with murine, but not with human, sIL-4R during the first week of infection rendered BALB/c mice clinically resistant to L. major, led to a 7- to 12-fold reduction of the parasite load in spleen and lymph nodes at 7 weeks of infection, shifted the pattern of cytokines towards a Th1 type, and provided durable resistance against reinfection. Thus, it could be demonstrated that the balance among sIL-4R, membrane-bound IL-4R, and their ligand IL-4 can be modulated in vivo, thereby modifying the antiparasitic immune response. These results suggest a therapeutic value of sIL-4R in diseases in which neutralization of IL-4 is desirable.

Links

PubMed PMC303084

Keywords

Animals; Base Sequence; Female; Interleukin-4/analysis; Interleukin-4/antagonists & inhibitors; Interleukin-4/genetics; Leishmaniasis, Cutaneous/immunology; Leishmaniasis, Cutaneous/therapy; Mice; Mice, Inbred BALB C; Molecular Sequence Data; RNA, Messenger/analysis; Receptors, Interleukin/analysis; Receptors, Interleukin/genetics; Receptors, Interleukin/physiology; Receptors, Interleukin-4; Recombinant Proteins/pharmacology; Recombinant Proteins/therapeutic use

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:IL4RA

acts_upstream_of_or_within

GO:0042832: defense response to protozoan

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:IL4

acts_upstream_of_or_within

GO:0042832: defense response to protozoan

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.