GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:7925459

From GONUTS
Jump to: navigation, search
Citation

Higashino, K, Ishizaki, J, Kishino, J, Ohara, O and Arita, H (1994) Structural comparison of phospholipase-A2-binding regions in phospholipase-A2 receptors from various mammals. Eur. J. Biochem. 225:375-82

Abstract

We determined the nucleotide sequence of a mouse cDNA encoding the receptor for pancreatic group I phospholipase A2 (PLA2-I). Interspecies structural comparison of the mouse receptor with bovine PLA2-I receptor, whose structure had been clarified, revealed that the fourth carbohydrate-recognition domain (CRD)-like domain (CRD-like 4) was the most conserved among the domains in the PLA2-I receptor, suggesting the functional importance of CRD-like 4. A transient expression experiment with a truncated form of the receptor consisting of three CRD-like domains, from the third to the fifth, demonstrated that the PLA2-I-binding site of the receptor is constituted from these three CRD-like domains, supporting the functional indispensability of CRD-like 4 in the receptor. Since the PLA2-I-binding region was thus assigned to be CRD-like domains 3-5, we further analyzed the structures of the PLA2-I-binding regions in the PLA2-I receptors from the rat, rabbit and human. Furthermore, the obtained PLA2-I receptor cDNA fragments from these animals made it possible to examine the tissue expression patterns of this receptor in various mammals. The results, together with the results of the genomic structural analysis of this gene, indicated that a PLA2 receptor recently characterized by Lambeau et al. [Lambeau, G., Ancian, P., Barhanin, J. & Lazdunski, M. (1994) J. Biol. Chem. 269, 1575-1578] is a rabbit counterpart of the PLA2-I receptor although these two PLA2 receptors have distinctive PLA2-binding specificities.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cattle; DNA Primers; Female; Fetus; Humans; Male; Mice; Molecular Sequence Data; Organ Specificity; Pancreas/metabolism; Phospholipases A/metabolism; Phospholipases A2; Pregnancy; RNA, Messenger/analysis; RNA, Messenger/biosynthesis; Rabbits; Rats; Receptors, Cell Surface/biosynthesis; Receptors, Cell Surface/chemistry; Receptors, Cell Surface/metabolism; Receptors, Phospholipase A2; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Restriction Mapping; Sequence Homology, Amino Acid; Species Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PIG:PA21B

enables

GO:0005102: signaling receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q62028

F

Seeded From UniProt

complete

MOUSE:PLA2R

involved_in

GO:0006898: receptor-mediated endocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PLA2R

enables

GO:0043274: phospholipase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P00592

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.