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PMID:7893679

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Citation

Rosario, MM, Kirby, JR, Bochar, DA and Ordal, GW (1995) Chemotactic methylation and behavior in Bacillus subtilis: role of two unique proteins, CheC and CheD. Biochemistry 34:3823-31

Abstract

We characterized mutants in two novel genes of Bacillus subtilis, cheC and cheD. Mutants in CheC had a high smooth swimming bias and exhibited poor adaptation to positive stimuli. Analysis of tethered cells revealed two distinct subpopulations which differ in their prestimulus bias and extent of adaptation. The receptors, the methyl-accepting chemotaxis proteins (MCPs), of this mutant strain were overmethylated, as a result of an increase in CheR activity. We speculate that CheC helps to control tumbling frequency by regulating CheR, perhaps by a feedback mechanism through the MCPs. In contrast, a cheD mutant exhibited very tumbly behavior, and many of the MCPs were unmethylated. It seems that some B. subtilis MCPs require the presence of CheD for CheR to methylate them, a unique feature of B. subtilis chemotaxis. It is hypothesized that CheD is part of a complex that facilitates methylation of some of the MCPs, and dissociation of CheD from this complex affects CheA activity and may help bring about adaptation.

Links

PubMed

Keywords

Bacillus subtilis/cytology; Bacterial Proteins; Chemotaxis/genetics; Cloning, Molecular; Escherichia coli/genetics; Genetic Complementation Test; Membrane Proteins/genetics; Membrane Proteins/physiology; Methylation; Methyltransferases/metabolism; Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BACSU:CHED

GO:0006935: chemotaxis

ECO:0000315:

P

See Results: Complementation of the cheD Null Mutant, and Capillary Assays of the cheD::cat Null Mutant.

complete
CACAO 10406

BACSU:CHEC

GO:0006935: chemotaxis

ECO:0000315:

P

See Results: Complementation of a cheC null mutant and Tethering Assays of the Null Mutants.

complete
CACAO 10407

Notes

See also

References

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