GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:7798260

From GONUTS
Jump to: navigation, search
Citation

Takahara, K, Lyons, GE and Greenspan, DS (1994) Bone morphogenetic protein-1 and a mammalian tolloid homologue (mTld) are encoded by alternatively spliced transcripts which are differentially expressed in some tissues. J. Biol. Chem. 269:32572-8

Abstract

Bone morphogenetic protein-1 (BMP-1) is a metalloprotease purified from extracts capable of inducing ectopic bone formation. In humans, it has a domain structure similar to that of the Drosophila dorsal-ventral patterning gene-product tolloid (Tld), but is considerably shorter. Here we show that, in humans and mice, alternatively spliced transcripts encode BMP-1 and a longer protein, designated mammalian tolloid (mTld), with a domain structure identical to that of Drosophila Tld. A third alternatively spliced product, in which a novel domain is inserted near the BMP-1 C terminus, is also reported. Low levels of transcripts for mTld were found in all adult human tissues surveyed, while BMP-1 transcripts were detectable in all adult tissues except brain. This differential expression was mirrored in embryonic mouse tissues where in situ hybridization found high levels of mTld transcripts, but was unable to detect BMP-1 transcripts, in the floor plate of the neural tube of the developing central nervous system. The third alternatively spliced form was not detected in adult human tissues. In situ hybridizations found punctate signals for all three forms localized to trophoblast giant cells in 17.5-day mouse placenta, with highest levels of expression, especially for BMP-1, near the maternal interface.

Links

PubMed

Keywords

Alternative Splicing; Animals; Base Sequence; Bone Morphogenetic Proteins; Cloning, Molecular; DNA, Complementary; Drosophila; Drosophila Proteins; Embryo, Mammalian/metabolism; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Growth Substances/genetics; Humans; Insect Hormones/genetics; Mice; Molecular Sequence Data; Placenta/metabolism; Proteins/genetics; RNA, Messenger/genetics; Sequence Homology, Amino Acid; Tolloid-Like Metalloproteinases

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:BMP1

involved_in

GO:0001501: skeletal system development

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP1

involved_in

GO:0007275: multicellular organism development

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP1

enables

GO:0008237: metallopeptidase activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.