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PMID:7744830
| Citation |
Sillman, AL and Monroe, JG (1995) Association of p72syk with the src homology-2 (SH2) domains of PLC gamma 1 in B lymphocytes. J. Biol. Chem. 270:11806-11 |
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| Abstract |
Phospholipase C gamma-catalyzed inositol phospholipid hydrolysis, a critical step in B cell antigen receptor signaling leading to second messenger generation and proliferation, depends upon tyrosine kinase activation. The B cell antigen receptor-associated tyrosine kinases p53/56lyn, p59fyn, p55blk, and p72syk are assumed to participate in receptor-initiated signaling. It is unknown, however, which of these kinases is involved in the tyrosine phosphorylation and resulting activation of phospholipase C gamma in response to antigen receptor cross-linking. We have used a fusion protein containing the tandem src homology-2 (SH2) domains of phospholipase C gamma 1 (PLC gamma 1) to identify B cell kinases which associate with PLC gamma 1. Using an in vitro kinase assay, we demonstrate SH2-dependent association of tyrosine kinase activity from anti-mu-stimulated B cells. The PLC gamma 1 SH2 domains associate with a prominent 70-72-kDa tyrosine phosphoprotein from anti-mu-stimulated, but not resting, B cells. Immunoblotting and secondary immunoprecipitation studies definitively identify this protein as p72syk. These results imply a physical interaction between PLC gamma 1 and p72syk in antigen receptor-stimulated B cells. This conclusion is confirmed by our ability to co-immunoprecipitate p72syk and PLC gamma 1 from lysates of anti-mu-stimulated B cells. These results implicate p72syk in the activation of phospholipase C gamma 1 during B cell antigen receptor signaling. |
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| Keywords |
Animals; B-Lymphocytes/metabolism; Enzyme Precursors/metabolism; Intracellular Signaling Peptides and Proteins; Isoenzymes/metabolism; Mice; Mice, Inbred BALB C; Phosphatidylinositols/metabolism; Phospholipase C gamma; Protein Structure, Tertiary; Protein-Tyrosine Kinases/metabolism; Receptors, Antigen, B-Cell/physiology; Recombinant Fusion Proteins/metabolism; Signal Transduction; Type C Phospholipases/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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