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PMID:7681220

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Citation

Brewster, JL, de Valoir, T, Dwyer, ND, Winter, E and Gustin, MC (1993) An osmosensing signal transduction pathway in yeast. Science 259:1760-3

Abstract

Yeast genes were isolated that are required for restoring the osmotic gradient across the cell membrane in response to increased external osmolarity. Two of these genes, HOG1 and PBS2, encode members of the mitogen-activated protein kinase (MAP kinase) and MAP kinase kinase gene families, respectively. MAP kinases are activated by extracellular ligands such as growth factors and function as intermediate kinases in protein phosphorylation cascades. A rapid, PBS2-dependent tyrosine phosphorylation of HOG1 protein occurred in response to increases in extracellular osmolarity. These data define a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment.

Links

PubMed

Keywords

Amino Acid Sequence; Blotting, Northern; Calcium-Calmodulin-Dependent Protein Kinases; Genes, Fungal; Molecular Sequence Data; Osmolar Concentration; Phosphorylation; Phosphothreonine/metabolism; Phosphotyrosine; Protein Kinases/chemistry; Protein Kinases/genetics; Restriction Mapping; Saccharomyces cerevisiae/genetics; Signal Transduction/genetics; Tyrosine/analogs & derivatives; Tyrosine/metabolism; Water-Electrolyte Balance/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:PBS2

involved_in

GO:0006972: hyperosmotic response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PBS2

enables

GO:0004708: MAP kinase kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P31938

F

Seeded From UniProt

complete

YEAST:PBS2

involved_in

GO:0007231: osmosensory signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PBS2

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:HOG1

involved_in

GO:0007231: osmosensory signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:HOG1

involved_in

GO:0006972: hyperosmotic response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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