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PMID:7677753

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Citation

Sato, H, Tsukada, T, Nagayoshi, M, Ikawa, Y, Aizawa, S and Kato, MV (1995) Repression of p53-dependent sequence-specific transactivation by MEF2c. Biochem. Biophys. Res. Commun. 214:468-74

Abstract

Lambda ZAP cDNA library constructed from spleen of a p53-deficient mouse was screened by South-Western technique using Fragment A, a DNA sequence that p53 specifically binds to, as a probe. One (clone 2) of six clones isolated was identical to MEF2c, a MADS-family transcription factor. Transcripts of the mef2c gene was also detected in spleen where the expression has not been reported so far. Isolated clones except clone 2 had a growth-suppression activity on p53-deficient osteosarcoma cell line (Saos II). Clone 2 repressed the transactivation from Fragment A by p53, suggesting that MEF2c may act as a negative regulator of p53-responsive element.

Links

PubMed Online version:10.1006/bbrc.1995.2310

Keywords

Animals; Base Sequence; Cell Line; Consensus Sequence; DNA, Complementary; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/metabolism; Gene Expression; Gene Library; Genes, p53; Humans; Immunoblotting; MADS Domain Proteins; Mice; Mice, Mutant Strains; Molecular Sequence Data; Myogenic Regulatory Factors; Oligonucleotide Probes; Recombinant Proteins/biosynthesis; Recombinant Proteins/metabolism; Spleen/metabolism; Transcription Factors/biosynthesis; Transcription Factors/metabolism; Transcription, Genetic; Transcriptional Activation; Transfection; Tumor Suppressor Protein p53/deficiency; Tumor Suppressor Protein p53/genetics; Tumor Suppressor Protein p53/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:P53

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:MEF2C

acts_upstream_of_or_within

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:98834

P

Seeded From UniProt

complete


See also

References

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