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PMID:7665913
| Citation |
Ozeki, H, Ito, S, Wakamatsu, K and Hirobe, T (1995) Chemical characterization of hair melanins in various coat-color mutants of mice. J. Invest. Dermatol. 105:361-6 |
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| Abstract |
Mammalian melanins exist in two chemically distinct forms: the brown to black eumelanins and the yellow to reddish pheomelanins. Melanogenesis is influenced by a number of genes, the levels of whose products determine the quantity and quality of the melanins produced. To examine the effects of various coat-color genes on the chemical properties of melanins synthesized in the follicular melanocytes of mice, we have introduced new methods to solubilize differentially pheomelanins and brown-type eumelanins. We applied these and previously developed high-performance liquid chromatography and spectrophotometric methods for assaying eu- and pheomelanins to characterize melanins in various mutant mice: black, lethal yellow, viable yellow, agouti, brown, light, albino, dilute, recessive yellow, pink-eyed dilution, slaty, and silver. It was demonstrated that 1) complete solubilization of melanins in Soluene-350 is a convenient method to estimate the total amount of eu- and pheomelanins, 2) lethal yellow, viable yellow, and recessive yellow hairs contain almost pure pheomelanins, and 3) melanins from brown, light, silver, and pink-eyed black hairs share chemical properties in common that are characterized by partial solubility in strong alkali. We suggest that 1) the brown-type eumelanins have lower degrees of polymerization than the black-type eumelanins, and 2) slaty hair melanin contains a greatly reduced ratio of 5,6-dihydroxyindole-2-carboxylic acid-derived units as compared with black and other eumelanic hair melanins. These results indicate that our methodology, high-performance liquid chromatography and spectrophotometric methods combined, may be useful in chemically characterizing melanin pigments produced in follicular melanocytes. |
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| Keywords |
Animals; Chromatography, High Pressure Liquid; Hair/metabolism; Hair Color/genetics; Melanins/chemistry; Melanins/metabolism; Mice/genetics; Mice, Inbred C57BL; Mice, Mutant Strains; Mutation; Spectrophotometry |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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