GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:7619207

From GONUTS
Jump to: navigation, search
Citation

Egerton, M, Needham, M, Evans, S, Millest, A, Cerillo, G, McPheat, J, Popplewell, M, Johnstone, D and Hollis, M (1995) Identification of multiple human calcitonin receptor isoforms: heterologous expression and pharmacological characterization. J. Mol. Endocrinol. 14:179-89

Abstract

The human breast carcinoma cell line T47D is known to express high-affinity calcitonin receptors (CTRs). PCR amplification of the CTR cDNA from T47D mRNA resulted in the identification of two different cDNAs that encode distinct receptor isoforms, h alpha CTR and h beta CTR. The two cDNAs are identical except that the h alpha CTR cDNA contains a 48 bp insert sequence that encodes a 16 amino acid domain in the first cytosolic loop of the receptor. Stable transfection of each receptor cDNA into murine erythroleukaemia (MEL) cells resulted in the expression of receptors with high affinity for radiolabelled salmon calcitonin (h alpha CTR Kd 0.09 nM, h beta CTR Kd 0.12 nM). Ligand competition binding studies did not reveal any significant pharmacological difference between the receptor isoforms. In transfected MEL cells and COS-1 cells the h beta CTR isoform was expressed at tenfold higher levels than the h alpha CTR. A reporter gene assay that monitored the coupling of CTR to adenylate cyclase by increases in beta-galactosidase activity indicated that both receptors were able to stimulate cyclic AMP production in response to ligand binding.

Links

PubMed

Keywords

Adenylate Cyclase/metabolism; Amino Acid Sequence; Amyloid/metabolism; Animals; Base Sequence; Binding, Competitive; Breast Neoplasms/metabolism; Breast Neoplasms/pathology; Calcitonin/metabolism; Calcitonin Gene-Related Peptide/metabolism; Carcinoma/metabolism; Carcinoma/pathology; Cyclic AMP/biosynthesis; DNA, Complementary/genetics; DNA, Neoplasm/genetics; Enzyme Activation/drug effects; Female; Gene Expression Regulation; Genes, Reporter; Humans; Islet Amyloid Polypeptide; Leukemia, Erythroblastic, Acute/pathology; Mice; Molecular Sequence Data; Neoplasm Proteins/biosynthesis; Neoplasm Proteins/genetics; Protein Conformation; Receptors, Calcitonin/classification; Receptors, Calcitonin/drug effects; Receptors, Calcitonin/genetics; Receptors, Calcitonin/metabolism; Second Messenger Systems/drug effects; Tumor Cells, Cultured

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CALCR

located_in

GO:0005887: integral component of plasma membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CALCR

involved_in

GO:0007189: adenylate cyclase-activating G protein-coupled receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CALCR

part_of

GO:0005887: integral component of plasma membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.