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PMID:7600576

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Citation

Berthold, J, Bauer, MF, Schneider, HC, Klaus, C, Dietmeier, K, Neupert, W and Brunner, M (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81:1085-93

Abstract

We have identified a complex in mitochondria that functions as a part of the preprotein import machinery of the inner membrane (MIM complex). Two known components, MIM23 and MIM17, and two novel components, MIM33 and MIM14, were found as constituents of this complex. In the presence of a translocating chain, the outer membrane import machinery (MOM complex) and the MIM complex form translocation contact sites. On the matrix side, the MIM complex is associated with the mt-Hsp70-MIM44 system. We propose a structure of the import machinery in which the MIM complex constitutes a proteinaceous channel that accepts preproteins from the MOM complex, facilitates their reversible transmembrane movement, and mediates unidirectional transport by linkage to the ATP-dependent mt-Hsp70-MIM44 system.

Links

PubMed

Keywords

Adenosine Triphosphate/metabolism; Base Sequence; Carrier Proteins/metabolism; Cloning, Molecular; DNA Primers; Escherichia coli; Fungal Proteins/biosynthesis; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; Genome, Fungal; HSP70 Heat-Shock Proteins/metabolism; Intracellular Membranes/metabolism; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Membrane Transport Proteins; Mitochondria/metabolism; Mitochondrial Membrane Transport Proteins; Molecular Sequence Data; Polymerase Chain Reaction/methods; Protein Precursors/biosynthesis; Protein Precursors/isolation & purification; Protein Precursors/metabolism; Protein Processing, Post-Translational; Repressor Proteins; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:TIM23

involved_in

GO:0030150: protein import into mitochondrial matrix

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:TIM23

part_of

GO:0005744: TIM23 mitochondrial import inner membrane translocase complex

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000003679

C

Seeded From UniProt

complete

YEAST:TIM17

involved_in

GO:0030150: protein import into mitochondrial matrix

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:TIM17

part_of

GO:0005744: TIM23 mitochondrial import inner membrane translocase complex

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000005300

C

Seeded From UniProt

complete


See also

References

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