PMID:7600576

Citation	Berthold, J, Bauer, MF, Schneider, HC, Klaus, C, Dietmeier, K, Neupert, W and Brunner, M (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81:1085-93
Abstract	We have identified a complex in mitochondria that functions as a part of the preprotein import machinery of the inner membrane (MIM complex). Two known components, MIM23 and MIM17, and two novel components, MIM33 and MIM14, were found as constituents of this complex. In the presence of a translocating chain, the outer membrane import machinery (MOM complex) and the MIM complex form translocation contact sites. On the matrix side, the MIM complex is associated with the mt-Hsp70-MIM44 system. We propose a structure of the import machinery in which the MIM complex constitutes a proteinaceous channel that accepts preproteins from the MOM complex, facilitates their reversible transmembrane movement, and mediates unidirectional transport by linkage to the ATP-dependent mt-Hsp70-MIM44 system.
Links	PubMed
Keywords	Adenosine Triphosphate/metabolism; Base Sequence; Carrier Proteins/metabolism; Cloning, Molecular; DNA Primers; Escherichia coli; Fungal Proteins/biosynthesis; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; Genome, Fungal; HSP70 Heat-Shock Proteins/metabolism; Intracellular Membranes/metabolism; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Membrane Transport Proteins; Mitochondria/metabolism; Mitochondrial Membrane Transport Proteins; Molecular Sequence Data; Polymerase Chain Reaction/methods; Protein Precursors/biosynthesis; Protein Precursors/isolation & purification; Protein Precursors/metabolism; Protein Processing, Post-Translational; Repressor Proteins; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:TIM23	involved_in	GO:0030150: protein import into mitochondrial matrix	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
YEAST:TIM23	part_of	GO:0005744: TIM23 mitochondrial import inner membrane translocase complex	ECO:0000353: physical interaction evidence used in manual assertion	SGD:S000003679	C		Seeded From UniProt	complete
YEAST:TIM17	involved_in	GO:0030150: protein import into mitochondrial matrix	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
YEAST:TIM17	part_of	GO:0005744: TIM23 mitochondrial import inner membrane translocase complex	ECO:0000353: physical interaction evidence used in manual assertion	SGD:S000005300	C		Seeded From UniProt	complete

References

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