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PMID:7594610

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Citation

Yount, NY, Wang, MS, Yuan, J, Banaiee, N, Ouellette, AJ and Selsted, ME (1995) Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis. J. Immunol. 155:4476-84

Abstract

Defensins constitute a family of 3- to 4-kDa antimicrobial peptides that are stored in the cytoplasmic granules of neutrophils, some macrophages, and intestinal Paneth cells. We have assessed defensin gene expression during myeloid differentiation by first characterizing cDNAs for each of the four known rat neutrophil defensins (RatNP 1-4). The cDNA sequences revealed that the peptides are synthesized as 87-94 amino acid precursors, each containing signal, pro-, and mature peptide segments. RatNP-3 and -4 mRNAs, but not those for RatNP-1 and -2 or other myeloid defensins, contained unique polypurine tracts located close to the termination codon in the 3' untranslated region. By using cDNA probes and/or riboprobes, we evaluated defensin transcript levels in a variety of tissues and in the full spectrum of neutrophil precursors. By in situ hybridization, defensin mRNAs were localized to neutrophil precursors in the bone marrow, with the highest mRNA levels occurring in promyelocytes and somewhat lower signals occurring in myeloblasts and myelocytes. Defensin mRNAs were not detectable in bands or mature neutrophils, nor at significant levels in tissues other than bone marrow. The accumulation of defensin protein in bone marrow cells was assessed by immunohistochemical staining with anti-RatNP-1 Ab. RatNP 1-4 mRNAs and protein levels were then correlated for each stage of neutrophilic differentiation to reveal the maturational profile of myeloid defensin gene expression in the rat.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Anti-Infective Agents/isolation & purification; Base Sequence; Blood Proteins/genetics; Blood Proteins/isolation & purification; Bone Marrow/chemistry; Bone Marrow/metabolism; Bone Marrow Cells; Cloning, Molecular; DNA, Complementary/isolation & purification; Defensins; Hematopoiesis; Male; Molecular Sequence Data; Neutrophils/chemistry; Organ Specificity; RNA, Messenger/metabolism; Rats; Rats, Sprague-Dawley

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:DEF4

located_in

GO:0005737: cytoplasm

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

RAT:DEF2

involved_in

GO:0042742: defense response to bacterium

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

RAT:DEF4

involved_in

GO:0006952: defense response

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

RAT:DEF4

part_of

GO:0005737: cytoplasm

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete


See also

References

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