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PMID:7588285

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Citation

Albrandt, K, Brady, EM, Moore, CX, Mull, E, Sierzega, ME and Beaumont, K (1995) Molecular cloning and functional expression of a third isoform of the human calcitonin receptor and partial characterization of the calcitonin receptor gene. Endocrinology 136:5377-84

Abstract

We have cloned and expressed two isoforms of the human calcitonin (hCT) receptor. Primers designed from the published sequence of a CT receptor cloned from an ovarian small cell carcinoma line were used for the polymerase chain reaction amplification of related products from human breast carcinoma MCF-7 cells. Two complementary DNAs were isolated. One clone lacks a 16-amino acid insert in the first intracellular loop and is virtually identical to the receptor recently cloned from the T47D human breast carcinoma cell line. The second clone is another splice variant lacking both the 16-amino acid insert in the first intracellular domain as well as the first 47 amino acids of the amino-terminus extracellular domain. COS-7 cells transfected with either receptor isoform bound [125I]salmon CT with high affinity and responded to hCT with increases in cAMP. Tissue distribution studies revealed the truncated extracellular domain 1 isoform transcripts in human skeletal muscle, kidney, brain, and lung. Analysis of a hCT receptor genomic clone demonstrated an exon/intron organization similar to that of the porcine CT receptor gene, except for a distinct exon coding for the alternatively spliced insert in the first intracellular domain.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Cells, Cultured; Cloning, Molecular; Cyclic AMP/biosynthesis; Exons; Humans; Introns; Molecular Sequence Data; Organ Specificity; Radioligand Assay; Receptors, Calcitonin/analysis; Receptors, Calcitonin/genetics; Receptors, Calcitonin/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CALCR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q91157

F

Seeded From UniProt

complete

HUMAN:CALCR

located_in

GO:0005887: integral component of plasma membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

ONCGO:Q91157

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P30988-3

F

Seeded From UniProt

complete

ONCGO:Q91157

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P30988-1

F

Seeded From UniProt

complete

ONCGO:Q91157

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P30988

F

Seeded From UniProt

complete

HUMAN:CALCR

involved_in

GO:0045762: positive regulation of adenylate cyclase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CALCR

enables

GO:0032841: calcitonin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q91157

F

Seeded From UniProt

complete

HUMAN:CALCR

part_of

GO:0005887: integral component of plasma membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CALCR

enables

GO:0004948: calcitonin receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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