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PMID:7568152

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Citation

Stanbrough, M, Rowen, DW and Magasanik, B (1995) Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes. Proc. Natl. Acad. Sci. U.S.A. 92:9450-4

Abstract

We have isolated the NIL1 gene, whose product is an activator of the transcription of nitrogen-regulated genes, by virtue of the homology of its zinc-finger domain to that of the previously identified activator, the product of GLN3. Disruption of the chromosomal NIL1 gene enabled us to compare the effects of Gln3p and of Nil1p on the expression of the nitrogen-regulated genes GLN1, GDH2, and GAP1, coding respectively for glutamine synthetase, NAD-linked glutamate dehydrogenase, and general amino acid permease. Our results show that the nature of GATAAG sequence that serve as the upstream activation sequence elements for these genes determines their abilities to respond to Gln3p and Nil1p. The results further indicate that Gln3p is inactivated by an increase in the intracellular concentration of glutamine and that Nil1p is inactivated by an increase in intracellular glutamate.

Links

PubMed PMC40819

Keywords

Amino Acid Sequence; Amino Acid Transport Systems; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; GATA Transcription Factors; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal/genetics; Glutamate Dehydrogenase/biosynthesis; Glutamate-Ammonia Ligase/biosynthesis; Glutamine/metabolism; Membrane Transport Proteins/biosynthesis; Molecular Sequence Data; Repressor Proteins; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:GLN3

involved_in

GO:0001080: nitrogen catabolite activation of transcription from RNA polymerase II promoter

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:GAT1

involved_in

GO:0001080: nitrogen catabolite activation of transcription from RNA polymerase II promoter

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:GAT1

involved_in

GO:0001080: nitrogen catabolite activation of transcription from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000842

P

Seeded From UniProt

complete

YEAST:GAT1

enables

GO:0043565: sequence-specific DNA binding

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000842

F

Seeded From UniProt

complete

YEAST:GAT1

involved_in

GO:0090294: nitrogen catabolite activation of transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:GAT1

involved_in

GO:0090294: nitrogen catabolite activation of transcription

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000842

P

Seeded From UniProt

complete


See also

References

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