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PMID:7559382

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Citation

Park, CH, Bessho, T, Matsunaga, T and Sancar, A (1995) Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease. J. Biol. Chem. 270:22657-60

Abstract

A complex, which consists of ERCC1 (38 kDa) and a 112-kDa protein, was purified from HeLa cells to homogeneity. This complex complemented the nucleotide excision repair defects of rodent ERCC-1, ERCC-4, and human XP-F mutant cell-free extracts, indicating that the 112-kDa protein is XPF/ERCC4 and providing direct biochemical evidence that XPF and ERCC4 are identical. The XPF/ERCC4-ERCC1 complex has an endonuclease activity with preference for single-stranded DNA and a single-stranded region of duplex DNA with a "bubble" structure. This complex also nicks supercoiled DNA weakly, and this nicking activity is stimulated by human replication protein A when the DNA contains UV damage.

Links

PubMed

Keywords

Base Sequence; Chromatography, Affinity; DNA Repair; DNA-Binding Proteins/isolation & purification; DNA-Binding Proteins/metabolism; Electrophoresis, Polyacrylamide Gel; Endonucleases; HeLa Cells; Humans; Molecular Sequence Data; Molecular Weight; Oligodeoxyribonucleotides; Proteins/isolation & purification; Proteins/metabolism; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ERCC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92889

F

Seeded From UniProt

complete

HUMAN:ERCC1

part_of

GO:0000109: nucleotide-excision repair complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ERCC1

contributes_to

GO:0000014: single-stranded DNA endodeoxyribonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:XPF

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P07992

F

Seeded From UniProt

complete


See also

References

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