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PMID:7557451

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Citation

Pichaud, F, Frendo, JL, Delage-Mourroux, R, de Vernejoul, MC, Moukhtar, MS and Jullienne, A (1995) Sequence of a novel mRNA coding for a C-terminal-truncated form of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. Gene 162:319-22

Abstract

We amplified, using the polymerase chain reaction (PCR) and NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (type-I 15-PGDH)-specific primers, RNA extracted from the HL-60 cell line. Two bands, differing in size by approx. 160 bp, were detected with ethidium bromide staining after electrophoresis of amplification products and hybridization with a 15-PGDH-specific probe. Sequencing these DNA bands revealed that the largest corresponded to the 15-PGDH cloned from human placenta [Ensor et al., J. Biol. Chem. 265 (1990) 14888-14891]. The smaller sequence coded for a predicted C-terminal-truncated form of 15-PGDH. This subtype of the type-I 15-PGDH mRNA was also found using RT-PCR in human liver, placenta and a cell line derived from a human medullary thyroid carcinoma (TT cells). Hybridization studies using specific probes indicated that this new mRNA form probably corresponded to the 3.4-kb mRNA, one of the two 15-PGDH mRNAs previously detected in Northern blot analysis.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Complementary/genetics; Gene Expression; HL-60 Cells; Humans; Hydroxyprostaglandin Dehydrogenases/genetics; Isoenzymes/genetics; Molecular Sequence Data; RNA, Messenger/genetics; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PGDH

enables

GO:0016404: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MRP4

enables

GO:0016404: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

See also

References

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