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PMID:7538907

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Citation

Chinnaiyan, AM, O'Rourke, K, Tewari, M and Dixit, VM (1995) FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell 81:505-12

Abstract

Using the cytoplasmic domain of Fas in the yeast two-hybrid system, we have identified a novel interacting protein, FADD, which binds Fas and Fas-FD5, a mutant of Fas possessing enhanced killing activity, but not the functionally inactive mutants Fas-LPR and Fas-FD8. FADD contains a death domain homologous to the death domains of Fas and TNFR-1. A point mutation in FADD, analogous to the lpr mutation of Fas, abolishes its ability to bind Fas, suggesting a death domain to death domain interaction. Overexpression of FADD in MCF7 and BJAB cells induces apoptosis, which, like Fas-induced apoptosis, is blocked by CrmA, a specific inhibitor of the interleukin-1 beta-converting enzyme. These findings suggest that FADD may play an important role in the proximal signal transduction of Fas.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Antigens, CD95; Antigens, Surface/genetics; Antigens, Surface/metabolism; Apoptosis; Base Sequence; Binding Sites/genetics; Carrier Proteins/genetics; Carrier Proteins/isolation & purification; Carrier Proteins/metabolism; Cell Line; Cloning, Molecular; Fas-Associated Death Domain Protein; Humans; Molecular Sequence Data; Point Mutation; Saccharomyces cerevisiae; Serpins/pharmacology; Signal Transduction; Viral Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TNR6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q13158

F

Seeded From UniProt

complete

HUMAN:FADD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P25445

F

Seeded From UniProt

complete

HUMAN:FADD

GO:0016265: death

ECO:0000316:

UniProtKB:P25445 UniProtKB:Q13158


P

Figures 4 and 5

complete
CACAO 3545

HUMAN:FADD

involved_in

GO:0008625: extrinsic apoptotic signaling pathway via death domain receptors

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FADD

enables

GO:0005123: death receptor binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FADD

GO:0031264:

ECO:0000247:

PMID:7538907[1]


Analysis of data using Northern blotting, sequence alignment and expression vectors

complete
CACAO 9344


See also

References

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  1. Chinnaiyan, AM et al. (1995) FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell 81 505-12 PubMed GONUTS page