GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:7503749

From GONUTS
Jump to: navigation, search
Citation

Rehli, M, Krause, SW, Schwarzfischer, L, Kreutz, M and Andreesen, R (1995) Molecular cloning of a novel macrophage maturation-associated transcript encoding a protein with several potential transmembrane domains. Biochem. Biophys. Res. Commun. 217:661-7

Abstract

Differentiation of tissue macrophages from bone marrow progenitor cells via circulating blood monocytes is a complex and multistep process. In order to analyze macrophage maturation on the molecular level we used the method of mRNA differential display to identify novel genes that are preferentially expressed in mature, in vitro differentiated macrophages. A PCR-fragment could be isolated that was amplified from macrophages, but not from freshly isolated monocytes, and macrophage-specific expression was confirmed by Northern blot analysis. Several corresponding cDNA clones could be isolated from a macrophage cDNA library, covering a nucleotide sequence of 2.5 kb. Nucleotide sequence of this cDNA showed no homology to known genes. The sequence reveals an open reading frame that codes for a 238 amino acid hydrophobic protein with seven potential transmembrane domains, suggesting a possible role as a receptor or an ion channel.

Links

PubMed Online version:10.1006/bbrc.1995.2825

Keywords

Amino Acid Sequence; Base Sequence; Cell Differentiation; Cells, Cultured; Cloning, Molecular; DNA Primers/chemistry; Gene Expression; Humans; Macrophages/cytology; Membrane Proteins/genetics; Molecular Sequence Data; Monocytes/cytology; Phosphoproteins/genetics; RNA, Messenger/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PAQRB

located_in

GO:0005887: integral component of plasma membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PAQRB

located_in

GO:0016020: membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PAQRB

part_of

GO:0016020: membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PAQRB

part_of

GO:0005887: integral component of plasma membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PAQRB

enables

GO:0038023: signaling receptor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.