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PMID:7499388

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Citation

Taira, E, Nagino, T, Taniura, H, Takaha, N, Kim, CH, Kuo, CH, Li, BS, Higuchi, H and Miki, N (1995) Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule. J. Biol. Chem. 270:28681-7

Abstract

We have cloned a novel cDNA of gicerin, a cell adhesion molecule belonging to the immunoglobulin superfamily. Both gicerin isoforms share the same extracellular domain, which has five immunoglobulin-like loop structures and a transmembrane domain as s-gicerin, but differ in the cytoplasmic tail domain. As the newly identified form has a larger cytoplasmic domain than the previously reported form, we refer to them as l-gicerin and s-gicerin, respectively. l-gicerin is transcribed from a distinct mRNA containing an inserted sequence not found in s-gicerin mRNA which caused a frameshift for the coding region for a cytoplasmic domain. Previous studies demonstrated that gicerin showed a doublet band of 82 and 90 kDa in chicken gizzard smooth muscle. We report that the 82-kDa protein corresponds to s-gicerin and the 90-kDa protein to l-gicerin. We also found that the two gicerin isoforms are expressed differentially in the developing nervous system. Functional analysis of these gicerin isoforms in stable transfectants revealed that they had differ in their homophilic adhesion properties, as well as in heterophilic cell adhesion assayed with neurite outgrowth factor. In addition, these isoforms have neurite-promoting activity by their homophilic adhesion, but differ in their ability to promote neurite outgrowth.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Antigens, CD146; Avian Proteins; Base Sequence; Blotting, Western; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/physiology; Cell Adhesion/genetics; Cell Adhesion Molecules/chemistry; Cell Adhesion Molecules/genetics; Cell Adhesion Molecules/physiology; Cell Aggregation/genetics; Cell Line; Chick Embryo; Chickens; DNA Primers/chemistry; DNA, Complementary; Ganglia/embryology; Genes, Immunoglobulin; Mice; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/physiology; Nervous System/embryology; Neurites; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:Q90880

involved_in

GO:0098743: cell aggregation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q90880

enables

GO:0098632: cell-cell adhesion mediator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q90880

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001945)
  • part_of:(UBERON:0002037)
  • part_of:(UBERON:0005052)

Seeded From UniProt

complete

CHICK:Q90880

involved_in

GO:0007156: homophilic cell adhesion via plasma membrane adhesion molecules

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q90880

involved_in

GO:0010976: positive regulation of neuron projection development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q90989

involved_in

GO:0098743: cell aggregation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q90989

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0000966)
  • part_of:(UBERON:0002037)
  • part_of:(UBERON:0005052)

Seeded From UniProt

complete

CHICK:Q90989

enables

GO:0098632: cell-cell adhesion mediator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q90989

involved_in

GO:0007156: homophilic cell adhesion via plasma membrane adhesion molecules

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q90989

involved_in

GO:0010976: positive regulation of neuron projection development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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