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PMID:7042346

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Citation

Ramos, F and Wiame, JM (1982) Occurrence of a catabolic L-serine (L-threonine) deaminase in Saccharomyces cerevisiae. Eur. J. Biochem. 123:571-6

Abstract

Saccharomyces cerevisiae mutants lacking the anabolic L-threonine deaminase, the ilv1- mutants, have been found to exhibit a normal ability to grow, without auxotrophy towards isoleucine, on L-threonine of L-serine as only nitrogen nutrient. Starting from a strain carrying a ilv1- mutation, a new mutation affecting the ability to utilize L-threonine as nitrogen source was selected. This mutation, which also impairs the ability to utilize L-serine, has been denominated cha-, for 'catabolism of hydroxyamino acids' and was found to result in the lack of a catabolic L-serine (L-threonine) deaminase. This enzyme which, unlike the anabolic threonine deaminase, is more active towards serine than towards threonine, differs from the latter enzyme by a number of biochemical and regulatory properties. Whereas the anabolic enzyme is an allosteric enzyme sensitive to feedback inhibition by isoleucine, the catabolic enzyme exhibits Michaelian kinetics: no control of its activity has been detected. Its synthesis is induced by L-serine and L-threonine. These two enzymes, which thus can be easily differentiated by means of their regulations, display a limited ability to compensate for one another's absence and appear to play clearly distinct roles under normal physiological conditions.

Links

PubMed

Keywords

L-Serine Dehydratase/isolation & purification; Mutation; Nitrogen/metabolism; Saccharomyces cerevisiae/enzymology; Threonine Dehydratase/isolation & purification

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:THDH

involved_in

GO:0006567: threonine catabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000569

P

Seeded From UniProt

complete

YEAST:STDH

involved_in

GO:0006567: threonine catabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000888

P

Seeded From UniProt

complete

YEAST:STDH

involved_in

GO:0006565: L-serine catabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000888

P

Seeded From UniProt

complete

YEAST:STDH

enables

GO:0004794: L-threonine ammonia-lyase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:STDH

enables

GO:0003941: L-serine ammonia-lyase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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