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PMID:6987663

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Citation

Glickman, BW and Radman, M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 77:1063-7

Abstract

Our approach to the isolation of DNA mismatch-correction-deficient mutants was based upon the isolation of 2-aminopurine-resistant second-site revertants of Escherichia coli dam- mutants. We isolated such second-site revertants which, when separated from the dam- mutation, have a mutator character of their own. These new mutators all mapped at three known mutator loci, mutH, mutL, and mutS, which exhibit the same mutagenic spectrum as the dam- mutator: increased levels of base substitution and frameshift mutations. The mutator potencies of double and triple mut- mutants suggest that these mutators are involved in the same general mismatch-repair pathway. All these mutations result in a hyper-recombination phenotype, but in four-factor crosses among lambda phages, a specific loss of intragenic recombination (Pam3 X Pam80) was found in mutL and mutS mutants, as would be predicted from the postulated role of mismatch correction in gene conversion and high negative interference phenomena.

Links

PubMed PMC348424

Keywords

2-Aminopurine/pharmacology; DNA Repair; DNA Replication; DNA, Bacterial/genetics; Drug Resistance, Microbial; Escherichia coli/genetics; Genes; Methylation; Mutation; Recombination, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MUTH

acts_upstream_of_or_within

GO:0006298: mismatch repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MUTH

part_of

GO:0032300: mismatch repair complex

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P23367
UniProtKB:P23909

C

Seeded From UniProt

complete

ECOLI:MUTL

acts_upstream_of_or_within

GO:0006298: mismatch repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MUTS

acts_upstream_of_or_within

GO:0006298: mismatch repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MUTL

involved_in

GO:0006298: mismatch repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MUTL

part_of

GO:0032300: mismatch repair complex

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P06722
UniProtKB:P23909

C

Seeded From UniProt

complete

ECOLI:MUTS

involved_in

GO:0006298: mismatch repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MUTS

part_of

GO:0032300: mismatch repair complex

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P06722
UniProtKB:P23367

C

Seeded From UniProt

complete


See also

References

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