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PMID:658175

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Citation

Sassa, S and Bernstein, SE (1978) Studies of erythrocyte protoporphyrin in anemic mutant mice: use of a modified hematofluorometer for the detection of heterozygotes for hemolytic disease. Exp. Hematol. 6:479-87

Abstract

Erythrocyte protoporphyrin concentration was determined on a single drop of blood from normal and anemic mutant mice using a modified hematofluorometer. Mice showed age- and sex-dependent changes in protoporphyrin concentrations. Mice with hypoplastic anemias had a slight elevation and mice with iron deficiency anemia had a moderate increase in red cell protoporphyrin. Those with inherited hemolytic anemias had a marked elevation of erythrocyte protoporphyrin. Mice heterozygous for hemolytic anemias had no overt hemolysis and reticulocytosis, yet their erythrocyte protoporphyrin concentrations were distinctly higher than their littermate controls. Previously these heterozygotes were differentiated only by laborious and expensive progeny testing. This paper is the first demonstration of a quick and practical way of detecting mice heterozygous for hemolytic anemias by erythrocyte protoporphyrin determination. The data of this study demonstrate not only the usefulness of the hematofluorometer for protoporphyrin determination in the diagnosis of mouse anemias, but also the significance of elevated erythrocyte protoporphyrin as a very sensitive indicator of increased erythropoiesis, or of iron deficiency.

Links

PubMed

Keywords

Anemia/blood; Anemia/genetics; Anemia, Hemolytic/blood; Anemia, Hemolytic/genetics; Anemia, Hypochromic/blood; Anemia, Hypochromic/genetics; Animals; Erythrocytes/analysis; Female; Fluorometry/instrumentation; Hematocrit; Heterozygote; Male; Mice; Mutation; Porphyrins/blood; Protoporphyrins/blood

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SPTA1

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856377

P

Seeded From UniProt

complete

MOUSE:SPTA1

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856181

P

Seeded From UniProt

complete

MOUSE:SPTB1

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856378

P

  • occurs_in:(CL:0000232)

Seeded From UniProt

complete

MOUSE:NRAM2

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856400

P

Seeded From UniProt

complete

MOUSE:ANK1

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856298

P

  • occurs_in:(CL:0000232)

Seeded From UniProt

complete

MOUSE:ANK1

acts_upstream_of_or_within

GO:0006779: porphyrin-containing compound biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856298

P

occurs_in:(CL:0000232)

Seeded From UniProt

complete


See also

References

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