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PMID:6338481

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Citation

Wower, I and Brimacombe, R (1983) The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane. Nucleic Acids Res. 11:1419-37

Abstract

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by reaction with 2-iminothiolane followed by a mild ultraviolet irradiation treatment. After removal of non-reacted protein and partial nuclease digestion of the cross-linked 16S RNA-protein moiety, a number of individual cross-linked complexes could be isolated and the sites of attachment of the proteins to the RNA determined. Protein S8 was cross-linked to the RNA at three different positions, within oligo-nucleotides encompassing positions 629-633, 651-654, and (tentatively) 593-597 in the 16S sequence. Protein S7 was cross-linked within two oligonucleotides encompassing positions 1238-1240, and 1377-1378. In addition, a site at position 723-724 was observed, cross-linked to protein S19, S20 or S21.

Links

PubMed PMC325806

Keywords

Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Escherichia coli/analysis; Imidoesters; Models, Molecular; Molecular Weight; Nucleic Acid Conformation; Protein Conformation; RNA, Ribosomal/analysis; Ribosomal Proteins/analysis; Ribosomes/analysis; Ribosomes/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:RS7

enables

GO:0019843: rRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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