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PMID:4105037

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Citation

Wright, M (1971) Mutants of Escherichia coli lacking endonuclease I, ribonuclease I, or ribonuclease II. J. Bacteriol. 107:87-94

Abstract

To isolate mutants of Escherichia coli K-12 lacking endonuclease I activity (end), a method has been developed which detects, by differential methyl green staining, undegraded deoxyribonucleic acid (DNA) in colonies previously incubated in toluene. This procedure allows isolation of mutant strains in which DNA degradation is reduced. For half of these strains, this defect has been correlated with deficiencies of endonuclease I, ribonuclease I (rns), or ribonuclease II (rne) activities. The enzymatic deficiencies of the other strains remain unknown. An rne mutation is cotransducible with serA (which is located at 56 min on the genetic map). Most end mutations, called endA, are also cotransducible with serA and are located between serA and strA. One end mutation, called endB, is located between purE and trp (i.e., between 13 and 25 min on the genetic map).

Links

PubMed PMC246889

Keywords

Cell-Free System; Chromosome Mapping; Crosses, Genetic; Culture Media; DNA, Bacterial/metabolism; Deoxyribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Genetics, Microbial; Hydrolysis; Mutagens; Mutation; Recombination, Genetic; Ribonucleases/metabolism; Rosaniline Dyes; Spectrophotometry; Staining and Labeling; Thymidine/metabolism; Toluene; Transduction, Genetic; Tritium

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:END1

acts_upstream_of_or_within

GO:0006308: DNA catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:END1

involved_in

GO:0006308: DNA catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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