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PMID:3967297

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Citation

Novick, P and Botstein, D (1985) Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell 40:405-16

Abstract

The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied. Both conditional-lethal actin mutants exhibit six phenotypes at the restrictive temperature: disruption of the asymmetric staining pattern of actin assembly; delocalized deposition of chitin on the cell surface; partial inhibition of secretion of the periplasmic protein, invertase; an intracellular accumulation of secretory vesicles; death of cells in the budded portion of the cell cycle upon prolonged incubation at the restrictive condition; and osmotic sensitivity. These results implicate actin in the organization and polarized growth of the yeast cell surface.

Links

PubMed

Keywords

Actins/genetics; Cell Cycle; Cell Survival; Chitin/analysis; Chromosomes; Glycoside Hydrolases/secretion; Mutation; Osmotic Pressure; Phenotype; Temperature; Yeasts/genetics; Yeasts/metabolism; beta-Fructofuranosidase

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ACT

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACT

located_in

GO:0000142: cellular bud neck contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACT

located_in

GO:0032432: actin filament bundle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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