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PMID:3915785

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Citation

Lemire, JM, Willcocks, T, Halvorson, HO and Bostian, KA (1985) Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 5:2131-41

Abstract

We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in Saccharomyces cerevisiae at the molecular level by analysis of previously isolated and genetically well-defined regulatory gene mutants known to affect APase expression. These mutants identify numerous positive- (PHO4, PHO2, PHO81) and negative-acting (PHO80, PHO85) regulatory loci dispersed throughout the yeast genome. We showed that the interplay of these positive and negative regulatory genes occurs before or during APase gene transcription and that their functions are all indispensible for normal regulation of mRNA synthesis. Biochemical evidence suggests that the regulatory gene products they encode are expressed constitutively. More detailed investigation of APase synthesis is a conditional PHO80(Ts) mutant indicated that neither PHO4 nor any other protein factor necessary for APase mRNA synthesis is transcriptionally regulated by PHO80. Moreover, in the absence of PHO80, the corepressor, presumed to be a metabolite of Pi, did not inhibit their function in the transcriptional activation of APase.

Links

PubMed PMC366931

Keywords

Acid Phosphatase/biosynthesis; Acid Phosphatase/genetics; Cycloheximide/pharmacology; Enzyme Repression; Genes; Genes, Fungal; Genes, Regulator; Genotype; Kinetics; Molecular Weight; Protein Biosynthesis/drug effects; RNA, Messenger/genetics; RNA, Messenger/isolation & purification; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:PHO2

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO2

involved_in

GO:0045937: positive regulation of phosphate metabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO4

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO4

involved_in

GO:0045937: positive regulation of phosphate metabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO85

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO85

involved_in

GO:0045936: negative regulation of phosphate metabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO80

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

YEAST:PHO80

involved_in

GO:0045936: negative regulation of phosphate metabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000296

P

Seeded From UniProt

complete

See also

References

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