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PMID:3821869

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Citation

Schneuwly, S, Klemenz, R and Gehring, WJ Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. Nature 325:816-8

Abstract

Genetic and molecular studies on the expression of Antennapedia (Antp) have suggested that this gene specifies mainly the second thoracic segment. On the basis of our molecular analysis of dominant gain-of-function mutants we have postulated that the transformation of antennae into second legs is due to the ectopic overexpression of the Antp+ protein. This hypothesis was tested by inserting the complementary DNA encoding the normal Antp protein into a heat-shock expression vector and subsequent germ-line transformation. As predicted, heat induction at defined larval stages leads to the transformation of antennae into second legs. The dorsal part of the head can also be transformed into second thoracic structures (scutum) indicating that Antp indeed specifies the second thoracic segment. By ectopic overexpression of the Antp protein the body plan of the fruit fly can be altered in a predictable way.

Links

PubMed Online version:10.1038/325816a0

Keywords

Animals; DNA, Recombinant; Drosophila/genetics; Extremities/embryology; Gene Expression Regulation; Head; Hot Temperature; Mutation; Thorax/embryology; Transformation, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ANTP

involved_in

GO:0007384: specification of segmental identity, thorax

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ANTP

involved_in

GO:0009948: anterior/posterior axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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