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PMID:368027
| Citation |
Thomson, J, Gerstenberger, PD, Goldberg, DE, Gociar, E, Orozco de Silva, A and Fraenkel, DG (1979) ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137:502-6 |
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| Abstract |
The Clarke-Carbon clone bank carrying ColE1-Escherichia coli DNA has been screened by conjugation for complementation of glycolysis and hexose monophosphate shunt mutations. Plasmids were identified for phosphofructokinase (pfkA), triose phosphate isomerase (tpi), phosphoglucose isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd), enolase (eno), phosphoglycerate kinase (pgk), and fructose-1,6-P2 aldolase (fda). Enzyme levels for the plasmid-carried gene ranged, for the various plasmids, from 4- to 25-fold the normal level. |
| Links | |
| Keywords |
Conjugation, Genetic; DNA, Bacterial/genetics; DNA, Recombinant; Escherichia coli/genetics; Genes; Genetic Complementation Test; Glycolysis; Hexosephosphates/metabolism; Mutation; Plasmids |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004347: glucose-6-phosphate isomerase activity |
ECO:0000315: |
F |
Table 3. A much higher than normal level of Phosphoglucose isomerase activity is conferred when a plasmid carrying pgi is introduced to the strain. |
complete | ||||
| GO:0003872: 6-phosphofructokinase activity |
ECO:0000315: |
F |
Table 2. A mutant strain that does not contain pfkA has a significantly lower phosphofructokinase activity than the wild type. |
complete | ||||
| GO:0004332: fructose-bisphosphate aldolase activity |
ECO:0000315: |
F |
Table 4. The overexpression of fda with a plasmid containing fda conferees a significantly higher amount of fructose-biphosphate aldolase activity. |
complete | ||||
| GO:0004807: triose-phosphate isomerase activity |
ECO:0000316: |
UniProtKB:P0A796
|
F |
Table 2. A mutant strain that has pfkA and tpi deleted has no triose-phophate isomerase activity. This is confirmed by another mutant that had only pfkA deleted and that had a normal level of triose-phosohate isomerase. |
complete | |||
| GO:0004618: phosphoglycerate kinase activity |
ECO:0000315: |
F |
Table 4. A mutant strain that does not contain pgk has significantly a significantly lower level of phosphoglycerate kinase activity than the wild type. |
complete | ||||
| GO:0004634: phosphopyruvate hydratase activity |
ECO:0000315: |
F |
Table 4. A mutant strain that does not contain eno has a significantly lower level of phosphopyruvate hydratase activity than the wild type. |
complete | ||||
| GO:0004616 : phosphogluconate dehydrogenase (decarboxylating) activity |
ECO:0000315: |
F |
Table 3. A mutant that contains gnd on a plasmid has a significantly higher phosphogluconate dehydrogenase activity than the wild type. |
complete | ||||
| GO:0004345: glucose-6-phosphate dehydrogenase activity |
ECO:0000315: |
F |
Table 3. A mutant strain that does not contain zwf has a significantly lower level of glucose-6-phosphate dehydrogenase activity than the wild type. |
complete | ||||
See also
References
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