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PMID:367782

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Citation

Henning, U, Sonntag, I and Hindennach, I (1978) Mutants (ompA) affecting a major outer membrane protein of Escherichia coli K12. Eur. J. Biochem. 92:491-8

Abstract

Seventy independent mutants have been analyzed affecting a major protein, polypeptide II, of the outer cell envelope membrane from Escherichia coli K12. They were classified as nonsense mutants of the amber type (20%), mutants most likely of the missense type possessing the protein at normal concentrations (9%), and mutants either missing the protein or harboring it at much reduced concentrations for unknown reasons (71%). Forty of the mutants were analyzed genetically and all were found to map at or near ompA, the structural gene for protein II. Two-dimensional electrophoretic analyses of envelopes from such mutants revealed an unusual heterogeneity of the protein which on such patterns appeared as at least 12 well separated spots, and the majority of these is due to artifacts of the method but apparently specific for this protein. In no case was a polypeptide fragment found in envelopes from the nonsense mutants. The results are discussed regarding two different phages which use the protein as a receptor and concerning the biosynthetic incorporation of the protein into the outer membrane.

Links

PubMed

Keywords

Cell Membrane/analysis; Coliphages; Escherichia coli/genetics; Genes; Membrane Proteins/genetics; Molecular Weight; Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:OMPA

acts_upstream_of_or_within

GO:0009597: detection of virus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:OMPA

acts_upstream_of_or_within

GO:0046718: viral entry into host cell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:OMPA

involved_in

GO:0009597: detection of virus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:OMPA

involved_in

GO:0046718: viral entry into host cell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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