GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:3531165

From GONUTS
Jump to: navigation, search
Citation

Crowell, DN, Anderson, MS and Raetz, CR (1986) Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli. J. Bacteriol. 168:152-9

Abstract

Several enzymes have been discovered recently in crude extracts of Escherichia coli that appear to be involved in the biosynthesis of the lipid A component of lipopolysaccharide. Two of these are lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase. Lipid A disaccharide synthase activity is barely detectable in cells harboring a lesion in the lpxB (pgsB) gene. We subcloned the lpxB gene from plasmid pLC26-43 of the Clarke and Carbon collection (L. Clarke and J. Carbon, Cell 9:91-99, 1976) and localized it to a 1.7-kilobase-pair fragment of DNA counterclockwise of dnaE on the E. coli chromosome. Furthermore, we discovered a new gene (lpxA) located adjacent to and counterclockwise of lpxB that encodes or controls UDP-N-acetylglucosamine acyltransferase. Our data prove that lpxB and lpxA are transcribed in the clockwise direction and suggest that they may be cotranscribed.

Links

PubMed PMC213431

Keywords

Acyltransferases/biosynthesis; Acyltransferases/genetics; Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Hexosyltransferases/biosynthesis; Hexosyltransferases/genetics; Lipid A/biosynthesis; N-Acetylglucosaminyltransferases; Plasmids; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:LPXB

acts_upstream_of_or_within

GO:0009245: lipid A biosynthetic process

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LPXB

involved_in

GO:0009245: lipid A biosynthetic process

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.