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PMID:3529090

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Citation

Giallongo, A, Feo, S, Moore, R, Croce, CM and Showe, LC (1986) Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase. Proc. Natl. Acad. Sci. U.S.A. 83:6741-5

Abstract

We previously purified a 48-kDa protein (p48) that specifically reacts with an antiserum directed against the 12 carboxyl-terminal amino acids of the c-myc gene product. Using an antiserum directed against the purified p48, we have cloned a cDNA from a human expression library. This cDNA hybrid-selects an mRNA that translates to a 48-kDa protein that specifically reacts with anti-p48 serum. We have isolated a full-length cDNA that encodes p48 and spans 1755 bases. The coding region is 1299 bases long; 94 bases are 5' noncoding and 359 bases are 3' noncoding. The cDNA encodes a 433 amino acid protein that is 67% homologous to yeast enolase and 94% homologous to the rat non-neuronal enolase. The purified protein has been shown to have enolase activity and has been identified to be of the alpha type by isoenzyme analysis. The transcriptional regulation of enolase expression in response to mitogenic stimulation of peripheral blood lymphocytes and in response to heat shock is also discussed.

Links

PubMed PMC386585

Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA/analysis; DNA/isolation & purification; Humans; Phosphopyruvate Hydratase/analysis; Phosphopyruvate Hydratase/genetics; Phosphopyruvate Hydratase/immunology; Proto-Oncogene Proteins/analysis; RNA, Messenger/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ENOA

part_of

GO:0000015: phosphopyruvate hydratase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ENOA

enables

GO:0042803: protein homodimerization activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:ENOA

involved_in

GO:0061621: canonical glycolysis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ENOA

enables

GO:0004634: phosphopyruvate hydratase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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