PMID:3094011

Citation	Cook, JS, Weldon, SL, Garcia-Ruiz, JP, Hod, Y and Hanson, RW (1986) Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken. Proc. Natl. Acad. Sci. U.S.A. 83:7583-7
Abstract	We have determined the sequence of the mRNA encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] from the chicken and have deduced the primary structure of the protein. The message for the enzyme is 2762 bases long and encodes a protein of 622 amino acids with a molecular mass of 69,522 daltons. The 5' untranslated region is 246 nucleotides long and contains two nonfunctional AUG initiator codons. The 3' untranslated sequence is 649 bases long and contains multiple polyadenylylation signals. There are regions of dyad symmetry and an A + U-rich region within the 3' translated and untranslated sequences of the message. Such regions are also present in the mRNA for the enzyme from the rat and may be of functional significance. Conserved regions of the enzyme, that may interact with substrates, were identified by comparing the amino acid sequence of phosphoenolpyruvate carboxykinase with that of other proteins that use guanine nucleotides and phosphoenolpyruvate as substrates.
Links	PubMed PMC386765
Keywords	Amino Acid Sequence; Animals; Base Sequence; Chickens; Cytosol/enzymology; GTP-Binding Proteins/analysis; Phosphoenolpyruvate Carboxykinase (GTP)/analysis; Phosphoenolpyruvate Carboxykinase (GTP)/genetics; Protein Conformation; RNA, Messenger/analysis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CHICK:PCKGC	involved_in	GO:0046898: response to cycloheximide	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0051384: response to glucocorticoid	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0051591: response to cAMP	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
CHICK:PCKGC	enables	GO:0004550: nucleoside diphosphate kinase activity	ECO:0000250: sequence similarity evidence used in manual assertion	UniProtKB:P07379	F		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0007568: aging	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0032496: response to lipopolysaccharide	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0032869: cellular response to insulin stimulus	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071320: cellular response to cAMP	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071300: cellular response to retinoic acid	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0070741: response to interleukin-6	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071347: cellular response to interleukin-1	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071356: cellular response to tumor necrosis factor	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	NOT|involved_in	GO:0018984: naphthalenesulfonate metabolic process	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
CHICK:PCKGC	enables	GO:0005525: GTP binding	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	F		Seeded From UniProt	complete
CHICK:PCKGC	enables	GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	F		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:1904640: response to methionine	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071456: cellular response to hypoxia	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071377: cellular response to glucagon stimulus	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0071332: cellular response to fructose stimulus	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete
CHICK:PCKGC	involved_in	GO:0033993: response to lipid	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P07379	P		Seeded From UniProt	complete

References

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