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PMID:3094011

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Citation

Cook, JS, Weldon, SL, Garcia-Ruiz, JP, Hod, Y and Hanson, RW (1986) Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken. Proc. Natl. Acad. Sci. U.S.A. 83:7583-7

Abstract

We have determined the sequence of the mRNA encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] from the chicken and have deduced the primary structure of the protein. The message for the enzyme is 2762 bases long and encodes a protein of 622 amino acids with a molecular mass of 69,522 daltons. The 5' untranslated region is 246 nucleotides long and contains two nonfunctional AUG initiator codons. The 3' untranslated sequence is 649 bases long and contains multiple polyadenylylation signals. There are regions of dyad symmetry and an A + U-rich region within the 3' translated and untranslated sequences of the message. Such regions are also present in the mRNA for the enzyme from the rat and may be of functional significance. Conserved regions of the enzyme, that may interact with substrates, were identified by comparing the amino acid sequence of phosphoenolpyruvate carboxykinase with that of other proteins that use guanine nucleotides and phosphoenolpyruvate as substrates.

Links

PubMed PMC386765

Keywords

Amino Acid Sequence; Animals; Base Sequence; Chickens; Cytosol/enzymology; GTP-Binding Proteins/analysis; Phosphoenolpyruvate Carboxykinase (GTP)/analysis; Phosphoenolpyruvate Carboxykinase (GTP)/genetics; Protein Conformation; RNA, Messenger/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:PCKGC

enables

GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0046015: regulation of transcription by glucose

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

enables

GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0046898: response to cycloheximide

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0051384: response to glucocorticoid

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0051591: response to cAMP

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0004550: nucleoside diphosphate kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P07379

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0007568: aging

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0032496: response to lipopolysaccharide

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0032869: cellular response to insulin stimulus

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071320: cellular response to cAMP

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071300: cellular response to retinoic acid

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0070741: response to interleukin-6

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071347: cellular response to interleukin-1

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071356: cellular response to tumor necrosis factor

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

NOT|involved_in

GO:0018984: naphthalenesulfonate metabolic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0005525: GTP binding

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

F

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:1904640: response to methionine

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071456: cellular response to hypoxia

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071377: cellular response to glucagon stimulus

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071332: cellular response to fructose stimulus

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0033993: response to lipid

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P07379

P

Seeded From UniProt

complete

Notes

See also

References

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