GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:3059494

From GONUTS
Jump to: navigation, search
Citation

Affholter, JA, Fried, VA and Roth, RA (1988) Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III. Science 242:1415-8

Abstract

A proteinase with high affinity for insulin has been proposed to play a role in the cellular processing of this hormone. A complementary DNA (cDNA) coding for this enzyme has been isolated and sequenced. The deduced amino acid sequence of the enzyme contained the sequences of 13 peptides derived from the isolated protein. The cDNA could be transcribed in vitro to yield a synthetic RNA that in cell-free translations produced a protein that coelectrophoresed with the native proteinase and could be immunoprecipitated with monoclonal antibodies to this enzyme. The deduced sequence of this proteinase did not contain the consensus sequences for any of the known classes of proteinases (that is, metallo, cysteine, aspartic, or serine), but it did show homology to an Escherichia coli proteinase (called protease III), which also cleaves insulin and is present in the periplasmic space. Thus, these two proteins may be members of a family of proteases that are involved in intercellular peptide signaling.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA/genetics; Endopeptidases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Humans; Insulysin/genetics; Metalloendopeptidases; Molecular Sequence Data; Peptide Hydrolases/genetics; Sequence Homology, Nucleic Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:IDE

involved_in

GO:0008286: insulin receptor signaling pathway

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.