GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:30462573

From GONUTS
Jump to: navigation, search
Citation

Rijal, R, Consalvo, KM, Lindsey, CK and Gomer, RH (2019) An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells. Mol. Biol. Cell 30:242-255

Abstract

Eukaryotic chemoattraction signal transduction pathways, such as those used by Dictyostelium discoideum to move toward cAMP, use a G protein-coupled receptor to activate multiple conserved pathways such as PI3 kinase/Akt/PKB to induce actin polymerization and pseudopod formation at the front of a cell, and PTEN to localize myosin II to the rear of a cell. Relatively little is known about chemorepulsion. We previously found that AprA is a chemorepellent protein secreted by Dictyostelium cells. Here we used 29 cell lines with disruptions of cAMP and/or AprA signal transduction pathway components, and delineated the AprA chemorepulsion pathway. We find that AprA uses a subset of chemoattraction signal transduction pathways including Ras, protein kinase A, target of rapamycin (TOR), phospholipase A, and ERK1, but does not require the PI3 kinase/Akt/PKB and guanylyl cyclase pathways to induce chemorepulsion. Possibly as a result of not using the PI3 kinase/Akt/PKB pathway and guanylyl cyclases, AprA does not induce actin polymerization or increase the pseudopod formation rate, but rather appears to inhibit pseudopod formation at the side of cells closest to the source of AprA.

Links

PubMed Online version:10.1091/mbc.E18-09-0562

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:PIAA

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RASG

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P32253

P

Seeded From UniProt

complete

DICDI:RASC

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P15064

P

Seeded From UniProt

complete

DICDI:KAPC

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RACC

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:CRAC

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:LST8

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Y1197

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:SCAR

acts_upstream_of_or_within

GO:0090630: activation of GTPase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:SCAR

involved_in

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:GRLH

acts_upstream_of_or_within

GO:0090630: activation of GTPase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54XY1

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAKD

acts_upstream_of_or_within

GO:0034260: negative regulation of GTPase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PTEN

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:IPLA

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ERK1

acts_upstream_of_or_within

GO:1903669: positive regulation of chemorepellent activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAKD

involved_in

GO:0034260: negative regulation of GTPase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:APRA

enables

GO:0005096: GTPase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DICDI:APRA

involved_in

GO:0090630: activation of GTPase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.