GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:3022150

From GONUTS
Jump to: navigation, search
Citation

Gros, P, Ben Neriah, YB, Croop, JM and Housman, DE Isolation and expression of a complementary DNA that confers multidrug resistance. Nature 323:728-31

Abstract

The emergence and outgrowth of a population of tumour cells resistant to multiple drugs is a major problem in the chemotherapeutic treatment of cancer. We have used highly drug-resistant cell lines developed in vitro to study the molecular basis of multidrug resistance. In these cell lines high levels of resistance are frequently associated with amplification and overexpression of a small group of genes termed mdr or gp170. Direct evaluation of the role of these genes in multidrug resistance has awaited the isolation of a member of this gene family in a biologically active form. Here we report the isolation of DNA clones complementary to the cellular messenger RNA transcripts of mdr genes and show that high-level expression of a full-length complementary DNA clone in an otherwise drug-sensitive cell confers a complete multidrug-resistant phenotype. Our results demonstrate that overexpression of a single member of the mdr group is sufficient to confer drug resistance. Furthermore, because the cDNA was isolated from a drug-sensitive cell, mutations in the primary sequence of mdr are not required to produce a multidrug-resistance phenotype.

Links

PubMed Online version:10.1038/323728a0

Keywords

Animals; Base Sequence; Cell Line; Cricetinae; DNA/isolation & purification; DNA Restriction Enzymes/metabolism; Drug Resistance/genetics; Gene Expression Regulation; Globins/genetics; Phenotype; RNA, Messenger/metabolism; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MDR1

located_in

GO:0016020: membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MDR1

involved_in

GO:0009410: response to xenobiotic stimulus

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MDR1

enables

GO:0042626: ATPase-coupled transmembrane transporter activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MDR1

enables

GO:0042626: ATPase activity, coupled to transmembrane movement of substances

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MDR1

involved_in

GO:0042493: response to drug

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MDR1

part_of

GO:0016020: membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MDR1

enables

GO:0005215: transporter activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.