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PMID:3019370

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Citation

Loewen, PC and Switala, J (1986) Purification and characterization of catalase HPII from Escherichia coli K12. Biochem. Cell Biol. 64:638-46

Abstract

Catalase (hydroperoxidase II or HPII) of Escherichia coli K12 has been purified using a protocol that also allows the purification of the second catalase HPI in large amounts. The purified HPII was found to have equal amounts of two subunits with molecular weights of 90,000 and 92,000. Only a single 92,000 subunit was present in the immunoprecipitate created when HPII antiserum was added directly to a crude extract, suggesting that proteolysis was responsible for the smaller subunit. The apparent native molecular weight was determined to be 532,000, suggesting a hexamer structure for the enzyme, an unusual structure for a catalase. HPII was very stable, remaining maximally active over the pH range 4-11 and retaining activity even in a solution of 0.1% sodium dodecyl sulfate and 7 M urea. The heme cofactor associated with HPII was also unusual for a catalase, in resembling heme d (a2) both spectrally and in terms of solubility. On the basis of heme-associated iron, six heme groups were associated with each molecule of enzyme or one per subunit.

Links

PubMed

Keywords

Catalase/isolation & purification; Catalase/metabolism; Escherichia coli/enzymology; Isoenzymes/isolation & purification; Isoenzymes/metabolism; Kinetics; Macromolecular Substances; Molecular Weight; Peroxidase; Peroxidases/isolation & purification; Peroxidases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:CATE

enables

GO:0004096: catalase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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