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PMID:2985560

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Citation

Burgess, DG and Penhoet, EE (1985) Characterization of the chicken aldolase B gene. J. Biol. Chem. 260:4604-14

Abstract

Vertebrates possess three isozyme forms of fructose diphosphate aldolase. We have isolated two overlapping chicken genomic clones which encode the liver-specific form of this enzyme; we have identified the 5' and 3' ends of this gene by a combination of primer extension analysis and S1 mapping; and we have determined the entire nucleotide sequence of this gene including 1400 base pairs (bp) of sequence from the regions flanking the 5' and 3' ends of the gene. The transcriptional unit for the aldolase B gene spans 8700 bp and contains eight intervening sequences, including a 4600-bp intron in the 5' non-coding region. On the basis of results from Southern genomic hybridizations, the aldolase B gene appears to be present only once per haploid genome. No differences were detected in the mRNA structure between RNA from three tissues expressing aldolase B (liver, kidney, and small intestine). Various features of the 5' flanking region are discussed, including a partial homology with the 5' noncoding region from rabbit aldolase A.

Links

PubMed

Keywords

Animals; Base Sequence; Brain/enzymology; Chickens; DNA Restriction Enzymes; Fructose-Bisphosphate Aldolase/genetics; Intestine, Small/enzymology; Isoenzymes/genetics; Kidney/enzymology; Liver/enzymology; Muscles/enzymology; Nucleic Acid Hybridization; RNA, Messenger/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:ALDOB

located_in

GO:0005815: microtubule organizing center

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

located_in

GO:0034451: centriolar satellite

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

located_in

GO:0070062: extracellular exosome

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0032781: positive regulation of ATP-dependent activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0004332: fructose-bisphosphate aldolase activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

part_of

GO:0005815: microtubule organizing center

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0006000: fructose metabolic process

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0006096: glycolytic process

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0006116: NADH oxidation

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0008092: cytoskeletal protein binding

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0030388: fructose 1,6-bisphosphate metabolic process

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0032781: positive regulation of ATPase activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

CHICK:ALDOB

part_of

GO:0034451: centriolar satellite

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0042802: identical protein binding

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0051117: ATPase binding

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0061609: fructose-1-phosphate aldolase activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

enables

GO:0070061: fructose binding

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

F

Seeded From UniProt

complete

CHICK:ALDOB

part_of

GO:0070062: extracellular exosome

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

C

Seeded From UniProt

complete

CHICK:ALDOB

involved_in

GO:0070072: vacuolar proton-transporting V-type ATPase complex assembly

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:P05062

P

Seeded From UniProt

complete

Notes

See also

References

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