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PMID:29588169

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Citation

'Pfab, A, Bruckmann, A, Nazet, J, Merkl, R and Grasser, KD (2018) The adaptor protein ENY2 is a component of the deubiquitination module of the Arabidopsis SAGA transcriptional co-activator complex but not of the TREX-2 complex. J. Mol. Biol. '

Abstract

The conserved nuclear protein ENY2 (Sus1 in yeast) is involved in coupling transcription and mRNA export in yeast and metazoa, as it is a component both of the transcriptional co-activator complex SAGA and of the mRNA export complex TREX-2. Arabidopsis thaliana ENY2 is widely expressed in the plant and it localises to the nucleoplasm, but unlike its yeast/metazoan orthologues it is not enriched in the nuclear envelope. Affinity purification of ENY2 in combination with mass spectrometry revealed that it co-purified with SAGA components, but not with the nuclear pore-associated TREX-2. In addition, further targeted proteomics analyses by reciprocal tagging established the composition of the Arabidopsis SAGA complex consisting of the four modules HATm, SPTm, TAFm and DUBm, and that several SAGA subunits occur in alternative variants. While the HATm, SPTm and TAFm robustly co-purified with each other, the de-ubiquitination module (DUBm) appears to associate with the other SAGA modules more weakly/dynamically. Consistent with a homology model of the Arabidopsis DUBm the SGF11 protein interacts directly with ENY2 and UBP22. Plants depleted in the DUBm components, SGF11 or ENY2, are phenotypically only mildly affected, but they contain increased levels of ubiquitinated histone H2B, indicating that the SAGA-DUBm has histone deubiquitination activity in plants. In addition to transcription-related proteins (i.e. transcript elongation factors, Mediator), many splicing factors were found to associate with SAGA, linking the SAGA complex and ongoing transcription with mRNA processing.

Links

PubMed Online version:10.1016/j.jmb.2018.03.018

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:B3H7K2

part_of

GO:0071819: DUBm complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT5G58575

C

Seeded From UniProt

complete

ARATH:B3H7K2

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT2G17930
AGI_LocusCode:AT3G27100
AGI_LocusCode:AT4G36080
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:B3H7K2

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT5G58575

C

Seeded From UniProt

complete

ARATH:B3H7K2

involved_in

GO:0035616: histone H2B conserved C-terminal lysine deubiquitination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4IPJ1

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT2G17930
AGI_LocusCode:AT3G27100
AGI_LocusCode:AT4G36080
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:F4JPL2

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT2G17930
AGI_LocusCode:AT3G27100
AGI_LocusCode:AT4G36080
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:SGF11

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT2G17930
AGI_LocusCode:AT3G27100
AGI_LocusCode:AT4G36080
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:SGF11

part_of

GO:0071819: DUBm complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT3G27100
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:SGF11

involved_in

GO:0035616: histone H2B conserved C-terminal lysine deubiquitination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:UBP22

part_of

GO:0070461: SAGA-type complex

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT2G17930
AGI_LocusCode:AT3G27100
AGI_LocusCode:AT4G36080
AGI_LocusCode:AT5G10790

C

Seeded From UniProt

complete

ARATH:ENY2

GO:0005654: nucleoplasm

ECO:0000314:

C

Figure (1m)shows nucleus of Arabidopsis thaliana and GFP expression of ENY2 shown evenly through the nucleoplasm and excluded from the nucleus.

complete
CACAO 13237

Notes

See also

References

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