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PMID:2942537

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Citation

Matson, SW (1986) Escherichia coli helicase II (urvD gene product) translocates unidirectionally in a 3' to 5' direction. J. Biol. Chem. 261:10169-75

Abstract

Escherichia coli helicase II, product of the uvrD gene, is a single-stranded DNA-dependent nucleoside 5'-triphosphatase with helicase activity. As a DNA-dependent ATPase, helicase II translocates processively along single-stranded DNA (S. W. Matson, unpublished results). The direction of translocation has been determined using a helicase assay that directly measures the ability of helicase II to catalyze the displacement of a labeled DNA fragment from one end of a single-stranded linear DNA molecule. The translocation of helicase II along single-stranded DNA is unidirectional and in the 3' to 5' direction with respect to the DNA strand on which the enzyme is bound. A kinetic analysis of the displacement of a labeled DNA fragment annealed to a linear single-stranded DNA molecule is also consistent with unidirectional translocation in the 3' to 5' direction. These results are contrary to results previously obtained using an indirect helicase assay (Kuhn, B., Abdel-Monem, M., Krell, H., and Hoffmann-Berling, H. (1979) J. Biol. Chem. 254, 11343-11350).

Links

PubMed

Keywords

Adenosine Triphosphatases/metabolism; Bacteriophages; DNA Helicases; DNA, Circular/metabolism; DNA, Single-Stranded/metabolism; DNA, Viral/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:UVRD

enables

GO:0017116: single-stranded DNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:UVRD

enables

GO:0003678: DNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:UVRD

enables

GO:0015616: DNA translocase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:UVRD

GO:0043138: 3'-5' DNA helicase activity

ECO:0000314:

F

Figure 3 shows that Helicase II translocates along single-stranded DNA in the 3' to 5' direction

complete

ECOLI:UVRD

GO:0003678: DNA helicase activity

ECO:0000314:

F

Figure 3 shows that Helicase II translocates along single-stranded DNA in the 3' to 5' direction

complete
CACAO 2039

ECOLI:UVRD

enables

GO:0017116: single-stranded DNA-dependent ATP-dependent DNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:UVRD

involved_in

GO:0032508: DNA duplex unwinding

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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