GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:2918403

From GONUTS
Jump to: navigation, search
Citation

Watford, M (1989) Hormonal and nutritional regulation of phosphoenolpyruvate carboxykinase mRNA levels in chicken kidney. J. Nutr. 119:319-22

Abstract

In chickens, the kidney possesses a distinct cytosolic phosphoenolpyruvate carboxykinase activity which is not found in the liver. This activity is subject to long-term regulation by diet and changes in acid-base status. The activity is increased during starvation or metabolic acidosis. In addition, an unidentified component of some standard chicken diets results in altered activity. Using a specific cDNA probe the abundance of PEPCK mRNA has been determined in chicken kidney in vivo and in vitro. The abundance of PEPCK mRNA in chicken kidney increases during starvation and is rapidly decreased after refeeding carbohydrate. In isolated kidney tubules the abundance of the mRNA is increased after incubation with glucocorticoids, dibutyryl cAMP or hormones acting via changes in the concentration of cAMP (parathyroid hormone, epinephrine). Phorbol esters or hormones acting via calcium-dependent mechanisms were without effect. The results support the hypothesis that in the chicken the kidney is the major site of gluconeogenesis from substrates other than lactate and thus plays an important role in the maintenance of glucose homeostasis.

Links

PubMed Online version:10.1093/jn/119.2.319

Keywords

Animal Nutritional Physiological Phenomena; Animals; Chickens/genetics; Chickens/metabolism; Dietary Carbohydrates/pharmacology; Gene Expression Regulation; Hormones/physiology; In Vitro Techniques; Kidney/enzymology; Male; Phosphoenolpyruvate Carboxykinase (GTP)/genetics; Phosphoenolpyruvate Carboxykinase (GTP)/metabolism; RNA, Messenger/isolation & purification; RNA, Messenger/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:PCKGC

involved_in

GO:0006111: regulation of gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0006111: regulation of gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0045722: positive regulation of gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0042594: response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071549: cellular response to dexamethasone stimulus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071374: cellular response to parathyroid hormone stimulus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071333: cellular response to glucose stimulus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0051591: response to cAMP

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0042149: cellular response to glucose starvation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0051379: epinephrine binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0042594: response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.