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PMID:28756655

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Citation

Song, C, Kim, T, Chung, WS and Lim, CO (2017) The Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions. Mol. Cells 40:577-586

Abstract

Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from , which was designated AtCYS5. RT-qPCR analysis showed that the expression of in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the -glucuronidase reporter gene under the control of the promoter showed that expression during seed germination was induced by HS and ABA. Constitutive overexpression of driven by the cauliflower mosaic virus promoter led to enhanced HS tolerance in transgenic , which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout () plants grown under HS conditions. The HS tolerance of -overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and . Although no HS elements were identified in the 5'-flanking region of , canonical ABA-responsive elements (ABREs) were detected. was upregulated in ABA-treated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of during seed germination and post-germination growth.

Links

PubMed PMC5582304 Online version:10.14348/molcells.2017.0075

Keywords

Abscisic Acid/pharmacology; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Basic-Leucine Zipper Transcription Factors/metabolism; Gene Expression Regulation, Developmental/drug effects; Gene Expression Regulation, Plant/drug effects; Germination/drug effects; Germination/genetics; Heat-Shock Response/drug effects; Heat-Shock Response/genetics; Plants, Genetically Modified; Promoter Regions, Genetic; Protein Binding/drug effects; Protoplasts/drug effects; Protoplasts/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Response Elements/genetics; Seedlings/drug effects; Seedlings/genetics; Seedlings/growth & development; Seeds/drug effects; Seeds/genetics; Seeds/growth & development; Transcription, Genetic/drug effects

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4I3C9

enables

GO:0043565: sequence-specific DNA binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT5G47550

F

Seeded From UniProt

complete

ARATH:CYT5

acts_upstream_of_or_within

GO:0010030: positive regulation of seed germination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CYT5

acts_upstream_of_or_within

GO:0034605: cellular response to heat

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AI5L6

enables

GO:0043565: sequence-specific DNA binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT5G47550

F

Seeded From UniProt

complete

Notes

See also

References

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