GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:2844725
| Citation |
Cary, JW, Petersen, DJ, Papoutsakis, ET and Bennett, GN (1988) Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli. J. Bacteriol. 170:4613-8 |
|---|---|
| Abstract |
A 13.6-kilobase (kb) Sau3AI restriction endonuclease fragment of Clostridium acetobutylicum DNA cloned into pBR322 enabled Escherichia coli ato mutants to grow on butyrate as a sole carbon source (But+). Complementation of the ato defect by the recombinant plasmid pJC6 was due to expression of the genes for phosphotransbutyrylase (PTB) and butyrate kinase (BK). Both genes were efficiently expressed in E. coli, as their products were readily detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell extracts. PTB was found to have a polypeptide subunit molecular weight of approximately 31,000, while that of BK was approximately 39,000. Deletion analysis and Tn5 mutagenesis of plasmid pJC7 (a But+ subclone containing a 4.4-kb BamHI fragment from the insert of pJC6) localized the PTB and BK genes within a region spanning approximately 2.9 kb. Preliminary evidence suggests that the two genes may form an operon that is transcribed as a single unit from a promoter of clostridial origin within the 4.4-kb insert of pJC7. |
| Links | |
| Keywords |
Acetyltransferases/genetics; Chromosome Mapping; Cloning, Molecular; Clostridium/enzymology; Clostridium/genetics; Escherichia coli; Gene Expression Regulation; Phosphate Acetyltransferase/genetics; Phosphate Acetyltransferase/metabolism; Phosphotransferases/genetics; Phosphotransferases/metabolism; Phosphotransferases (Carboxyl Group Acceptor); Restriction Mapping; Sequence Homology, Nucleic Acid |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0047761: butyrate kinase activity |
ECO:0000314: |
F |
Table 1 |
complete | ||||
| GO:0050182 : phosphate butyryltransferase activity |
ECO:0000314: |
F |
Table 1 |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
