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PMID:2828314

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Citation

Wilson, ML and Macnab, RM (1988) Overproduction of the MotA protein of Escherichia coli and estimation of its wild-type level. J. Bacteriol. 170:588-97

Abstract

The motA gene of Escherichia coli was placed under the control of a high-level promoter, that of the tryptophan operon of Serratia marcescens. In the presence of the inducer beta-indoleacrylic acid, MotA was synthesized at greatly elevated levels and inserted without apparent limit into the inner membrane. Growth and motility were impaired, but not drastically so, indicating that MotA by itself does not act as a proton ionophore. Antibody raised against the overproduced protein was used to estimate that a wild-type cell contained 600 +/- 250 copies of MotA. This number is more than would be needed to surround each flagellar basal body with a single circlet of MotA protein; possible interpretations of the result are discussed. The antibody was also used to establish that the MotA protein of Salmonella typhimurium has a similar molecular weight to that of E. coli and is immunologically cross-reactive with it; functional complementation of S. typhimurium motA mutants by the E. coli gene was established.

Links

PubMed PMC210695

Keywords

Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Cell Membrane/metabolism; Cell Movement; Cloning, Molecular; DNA Restriction Enzymes; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli/ultrastructure; Flagella; Gene Expression Regulation; Genes, Bacterial; Genetic Complementation Test; Membrane Proteins/biosynthesis; Membrane Proteins/genetics; Plasmids; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MOTA

located_in

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MOTA

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MOTA

acts_upstream_of_or_within

GO:0071973: bacterial-type flagellum-dependent cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MOTA

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MOTA

part_of

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MOTA

involved_in

GO:0071973: bacterial-type flagellum-dependent cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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